Single-cell transcriptomics reveals neural stem cell trans-differentiation and cell subpopulations in whole heart decellularized extracellular matrix  

作　　者：Xiaoning Yang Yuwei Zhao Wei Liu Zhongbao Gao Chunlan Wang Changyong Wang Siwei Li Xiao Zhang 

机构地区：[1]Beijing Institute of Basic Medical Sciences,Beijing 100850,China

出　　处：《Biophysics Reports》2024年第4期241-253,共13页生物物理学报（英文版）

基　　金：supported by Youth Talent Fund Project of Military Medical Research Institute(AMMS-QNPY-2021-005);the Joint Fund Project of the National Natural Science Foundation of China(U21A20394);National Natural Science Foundation of China Youth Fund(32201116).

摘　　要：The whole heart decellularized extracellular matrix(ECM)has become a promising scaffold material for cardiac tissue engineering.Our previous research has shown that the whole heart acellular matrix possesses the memory function regulating neural stem cells(NSCs)trans-differentiating to cardiac lineage cells.However,the cell subpopulations and phenotypes in the trans-differentiation of NSCs have not been clearly identified.Here,we performed single-cell RNA sequencing and identified 2,765 cells in the recellularized heart with NSCs revealing the cellular diversity of cardiac and neural lineage,confirming NSCs were capable of trans-differentiating into the cardiac lineage while maintaining the original ability to differentiate into the neural lineage.Notably,the trans-differentiated heart-like cells have dual signatures of neuroectoderm and cardiac mesoderm.This study unveils an in-depth mechanism underlying the trans-differentiation of NSCs and provides a new opportunity and theoretical basis for cardiac regeneration.

关 键 词：Single-cell transcriptomics Neural stem cell TRANS-DIFFERENTIATION Decellularized extracellular matrix Cardiac lineage cells 

分 类 号：R318[医药卫生—生物医学工程]