中国旱獭、土拨鼠肝组织及外周血单核细胞中Ⅰ型干扰素受体的表达情况  

Study on expression of type Ⅰ interferon receptor in liver and peripheral blood mononuclear cells of marmota himalayana and woodchuck

作　　者：张艳琼 刘艺 毛高峰 樊奕翔 桂文甲 陶颖 李智 杨东亮[3] 樊和斌 ZHANG Yanqiong;LIU Yi;MAO Gaofeng;FAN Yixiang;GUI Wenjia;TAO Ying;LI Zhi;YANG Dongliang;FAN Hebin(Department of Infection,General Hospital of the Central Theater Command,Wuhan 430030;Department of Neurology,General Hospital of the Central Theater Command;Department of Infection,Union Hospital,Tongji Medical College,Huazhong Universtiy of Science and Technology,China)

机构地区：[1]中国人民解放军中部战区总医院感染科,湖北武汉430030 [2]中国人民解放军中部战区总医院神经内科 [3]华中科技大学附属协和医院感染科

出　　处：《胃肠病学和肝病学杂志》2024年第10期1281-1285,共5页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的了解中国旱獭、土拨鼠肝组织及外周血单核细胞(peripheral blood mononuclear cells,PBMC)中Ⅰ型干扰素受体(type Ⅰ interferon receptor,mhIFNAR)的表达情况。方法采用荧光定量RT-PCR检测中国旱獭、土拨鼠肝组织、PBMC中mhIFNAR的表达情况及其与感染状态是否存在相关性,以及WH12-6、PWH细胞在α、γ干扰素刺激下mhIFNAR的表达情况。结果中国旱獭肝组织、PBMC中mhIFNAR表达,肝组织现症感染组及未感染组中mhIFNAR1和mhIFNAR2分别为3.9±2.6、6.2±3.90(P=0.405),2.97±1.25、49.58±34.53(P=0.064),PBMC中分别为:5.675±3.076、5.18±1.16(P=0.694),32.04±13.01、4.35±1.44(P=0.000)。α和γ干扰素可以使WH12-6细胞中mhIFNAR1和mhIFNAR2分别上调(3.002±6.788)倍、(42.51±5.42)倍,而γ干扰素分别上调(11.342±0.242)倍和(13.72±1.48)倍。土拨鼠不同感染状态下肝组织中mhIFNAR的表达如下:mhIFNAR1和mhIFNAR2在慢性感染组、急性感染组、未感染组及合并HDV感染组分别为2.658±0.988、6.928±6.678、7.516±4.391、20.481±7.985(P=0.001);mhIFNAR2则分别为4.202±1.983、11.951±10.587、8.075±1.310、30.750±6.408(P=0.000);PWH在α、γ干扰素刺激下,其中mhIFNAR2分别上调(1.55±0.193)倍及(2.015±0.145)倍。结论中国旱獭肝组织、PBMC可以检测到mhIFNAR的表达,且PBMC中mhIFNAR2的表达量与感染结局存在相关性,同样在土拨鼠肝组织内mhIFNAR的表达与感染状态存在相关性,慢性感染组偏低。而α、γ干扰素可以上调mhIFNAR的表达,为其抗病毒治疗的机制提供了新的依据。Objective To understand the expressions of mhIFNAR in liver tissues,peripheral blood mononuclear cells(PBMC)and the expression of mhIFNAR in WH12-6,PWH through the stimulation withα,γinterferon.Methods The expression of mhIFNAR in liver and PBMC were quantified by realtime RT-PCR.The WH12-6 and PWH cells were stimulated withα,γinterferon for 24 hours.And then the expression of mhIFNAR were also quantified by realtime RT-PCR.Results The mhIFNAR1 and mhIFNAR2 in liver tissues between current infection and uninfection were 3.9±2.6,6.2±3.90(P=0.405);2.97±1.25,49.58±34.53(P=0.064)respectively,and in PBMC were 5.675±3.076,5.18±1.16(P=0.694);32.04±13.01,4.35±1.44(P=0.000).When the WH12-6 cells were stimulatedαinterferon,mhIFNAR1 and mhIFNAR2 were(3.002±6.788)times,(42.51±5.42)times higher than unstimulated cells,γinterferon also enhanced(11.342±0.242)times and(13.72±1.48)times,respectively.The mhIFNAR1 in woodchuck liver tissues among chronic carriers,actue infection,uninfecion and cofection HDV groups were 2.658±0.988,6.928±6.678,7.516±4.391 and 20.481±7.985(P=0.001),the mhIFNAR2 were 4.202±1.983,11.951±10.587,8.075±1.310 and 30.750±6.408(P=0.000).When the PWH were stimulatedαandγinterferon,the mhIFNAR2 were(1.55±0.193)times and(2.015±0.145)times higher than unstimulated cells respectively.Conclusion The expression of mhIFNAR2 in PBMC is associated with the outcome of WHV infection in marmota himalayana,and the expression of mhIFNAR in liver of woodchuck is low in WHV infection group.Last theα,γinterferon can upregulate the mhIFNAR in WH12-6 and PWH cells.This may explain the reason why different outcome of WHV infection,and explain the antiviral mechanism ofα,γinterferon.

关 键 词：干扰素受体 肝组织 外周血单核细胞 中国旱獭 土拨鼠 

分 类 号：R575[医药卫生—消化系统]