强心胶囊对SD心力衰竭大鼠Wnt/β-catenin信号通路导致纤维化的抑制效果研究  

Study on inhibitory effect of Qiangxin Capsule on fibrosis caused by Wnt/β-catenin signaling pathway in SD heart failure rats

作　　者：曲扬 杜义斌 陈必勤 李黔云 QU Yang;DU Yibin;CHEN Biqin;LI Qianyun(First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming,Yunnan 650021,China)

机构地区：[1]云南中医药大学第一附属医院,昆明650021

出　　处：《重庆医学》2024年第19期2887-2891,共5页Chongqing Medical Journal

基　　金：云南省科技厅基础研究专项-青年项目(202001AU070127)。

摘　　要：目的研究强心胶囊对Sprague Dawley(SD)心力衰竭(心衰)大鼠Wnt(wingless)/β-连环蛋白(β-catenin)信号通路导致纤维化的抑制效果及相关机制。方法选取6~8周龄SPF级SD雄性大鼠15只作为研究对象。将大鼠随机分为假手术组、模型组、给药组,每组5只。模型组与给药组通过手术造成腹主动脉部分狭窄。假手术组大鼠和模型组大鼠给予生理盐水10 mL/kg灌胃,给药组大鼠给予强心胶囊0.8 g/kg灌胃,各组连续灌胃给药20周。采用室壁运动幅度检测各组大鼠室左心室射血分数(LVEF)、左心室收缩末内径(LVEDs)、左心室舒张末内径(LVEDd)。20周后处死各组大鼠,取大鼠心肌组织进行病理分析,苏木素-伊红(HE)染色观察心肌组织、Masson染色观察纤维组织,Western blot法检测Wnt、β-catenin、胶原蛋白Ⅰ和胶原蛋白Ⅲ的蛋白表达水平。结果给药组LVEF高于模型组,LVEDd、LVEDs小于模型组,差异均有统计学意义(P<0.05);与模型组对比,给药组Wnt/GAPDH、β-catenin/GAPDH降低,差异有统计学意义(P<0.05);与模型组对比,给药组胶原蛋白Ⅰ/GAPDH、胶原蛋白Ⅲ/GAPDH降低,差异有统计学意义(P<0.05)。结论强心胶囊可减轻腹主动脉缩窄后SD心衰大鼠的心脏纤维化程度,其机制可能与抑制Wnt/β-catenin信号通路有关。Objective To study the inhibitory effect and related mechanisms of Qiangxin Capsule on fibrosis caused by Wnt(wingless)/β-catenin signaling pathway in Sprague Dawley(SD)heart failure(HF)rats.Methods Fifteen SPF-grade SD male rats aged 6-8 weeks were selected as the research subjects.The rats were randomly divided into the sham operation group,model group and medication group,5 cases in each group.The model group and medication group caused abdominal aorta partial stenosis by operation.The rats in the sham operation group and model group were gavaged by 10 mL/kg of normal saline,the rats in the medication group were gavaged by 0.8 g/kg Qiangxin Capsule,and each group was continuously administered for 20 weeks.The ventricular wall motion amplitude was used to detect the left ventricular ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVEDs)and left ventricular end-diastolic diameter(LVEDd)in each group.After 20 weeks,the rats in each group were executed,the rat myocardial tissues were taken for conducting the pathological analysis,the myocardial tissues were observed by hematoxylin-eosin(HE)staining,the fibrous tissues were observed by the Masson staining,and the protein expression levels of Wnt,β-catenin,collagenⅠand collagenⅢwere detected by Western blot.Results LVEF in the medication group was higher than that in the model group,LVEDd and LVEDs were smaller than those in the model group,and the differences were statistically significant(P<0.05).Compared with the model group,the Wnt/GAPDH andβ-catenin/GAPDH levels in the medication group were decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the collagenⅠ/GAPDH and collagenⅢ/GAPDH in the medication group were decreased,and the difference was statistically significant(P<0.05).Conclusion Qiangxin Capsule could alleviate the cardiac fibrosis degree in SD rats with heart failure after abdominal aortal coarctation,and its mechanism may be related to the inhibition of Wnt/β-catenin si

关 键 词：强心胶囊 心力衰竭 温阳利水 心脏纤维化 WNT/Β-CATENIN 心室重构 

分 类 号：R-322[医药卫生]