Jianpi-Huatan-Huoxue-Anshen formula ameliorates gastrointestinal inflammation and microecological imbalance in chemotherapytreated mice transplanted with H22 hepatocellular carcinoma  

作　　者：Ya-Nan Wang Xiang-Yang Zhai Zheng Wang Chun-Ling Gao Sui-Cai Mi Wen-Li Tang Xue-Min Fu Huai-Bang Li Li-Feng Yue Peng-Fei Li Sheng-Yan Xi 

机构地区：[1]Department of TCM,Xiang’an Hospital,School of Medicine,Xiamen University,Xiamen 361102,Fujian Province,China [2]Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Macao 999078,China [3]Department of Radiotherapy,Chenggong Hospital of Xiamen University,PLA 73rd Army Hospital,Xiamen 361003,Fujian Province,China [4]Department of Oncology,Xiamen Hospital of Traditional Chinese Medicine,Xiamen 361015,Fujian Province,China [5]Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第10期4209-4231,共23页世界胃肠肿瘤学杂志（英文）

基　　金：Supported by Natural Science Foundation of Xiamen,China,No.3502Z20227171;the Young Investigator Research Program of Xiang’an Hospital of Xiamen University,No.XAH23005;the Traditional Chinese Medicine Foundation of Xiamen,No.XWZY-2023-0103;Natural Science Foundation of Fujian,China,No.2018J01136;National Natural Science Foundation of China,No.81202659.

摘　　要：BACKGROUND Jianpi-Huatan-Huoxue-Anshen formula[Tzu-Chi cancer-antagonizing&lifeprotecting II decoction(TCCL)]is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects of chemotherapy in cancer patients and improve their quality of life.However,its effect and mechanism on the intestinal microecology after chemotherapy are not yet clear.AIM To discover the potential mechanisms of TCCL on gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with hepatocellular carcinoma(HCC).METHODS Ninety-six mice were inoculated subcutaneously with HCC cells.One week later,the mice received a large dose of 5-fluorouracil by intraperitoneal injection to establish a HCC chemotherapy model.Thirty-six mice were randomly selected before administration,and feces,ileal tissue,and ileal contents were collected from each mouse.The remaining mice were randomized into normal saline,continuous chemotherapy,Yangzheng Xiaoji capsulestreated,and three TCCL-treated groups.After treatment,feces,tumors,liver,spleen,thymus,stomach,jejunum,ileum,and colon tissues,and ileal contents were collected.Morphological changes,serum levels of IL-1β,IL-6,IL-8,IL-10,IL-22,TNF-α,and TGF-β,intestinal SIgA,and protein and mRNA expression of ZO-1,NF-κB,Occludin,MUC-2,Claudin-1,and IκB-αin colon tissues were documented.The effect of TCCL on the abundance and diversity of intestinal flora was analyzed using 16S rDNA sequencing.RESULTS TCCL treatment improved thymus and spleen weight,thymus and spleen indexes,and body weight,decreased tumor volumes and tumor tissue cell density,and alleviated injury to gastric,ileal,and colonic mucosal tissues.Among proteins and genes associated with inflammation,IL-10,TGF-β,SIgA,ZO-1,MUC-2,and Occludin were upregulated,whereas NF-κB,IL-1β,IL-6,TNF-α,IL-22,IL-8,and IκB-αwere downregulated.Additionally,TCCL increased the proportions of fecal Actinobacteria,AF12,Adlercreutzia,Clostridium,Coriobacteriaceae,and Paraprevotella in the

关 键 词：Chinese medicine Chemotherapy H22 hepatocellular carcinoma Intestinal barrier function Intestinal microecological balance 

分 类 号：R735.7[医药卫生—肿瘤]