Intratumoural microorganism can affect the progression of hepatocellular carcinoma  

作　　者：Bao-Qun Liu Yi Bai Da-Peng Chen Ya-Min Zhang Tian-Ze Wang Jing-Rui Chen Xiang-Yu Liu Bin Zheng Zi-Lin Cui 

机构地区：[1]First Central Clinical College,Tianjin Medical University,Tianjin 300070,China [2]Department of Hepatobiliary Surgery,Tianjin First Central Hospital,School of Medicine,Nankai University,Tianjin 300192,China [3]Tianjin First Central Hospital Clinic Institute,School of Medicine,Nankai University,Tianjin 300192,China [4]School of Medicine,Tianjin University,Tianjin 300072,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第10期4232-4243,共12页世界胃肠肿瘤学杂志（英文）

基　　金：Supported by the Tianjin Health Science and Technology Project Surface Project,No.TJWJ2023MS012.

摘　　要：BACKGROUND Several recent studies have confirmed that intratumoural microorganisms can affect the occurrence and development of hepatocellular carcinoma(HCC);however,their role in tumor progression remains unclear.Hence,there is a need for further research on the role of intratumoural microorganisms in HCC.AIM To investigate the changes in intratumoural microorganisms in HCC and the effect of Propionibacterium on HCC progression.METHODS HCC and normal liver tissue specimens were subjected to fluorescence in situ hybridization(FISH).After performing 16S rRNA sequencing on HCC and peritumoral tissues to analyze the differences between the two groups.Propionibacterium was cocultured with HCC cells in vitro.Changes in cell proliferation and migration capacity were evaluated.The expression of NF-κB pathway related proteins in tumor cells was compared.The orthotopic liver implantation model and the subcutaneous xenograft model were constructed.liver tissues and subcutaneous tumors were collected 2 weeks later.RESULTS FISH demonstrated the presence of microorganisms in HCC and normal liver tissues.16S rRNA sequencing revealed an abundance of Lysobacter,Lachnospiraceae,Pseudomonas,and Lactobacillus in HCC tissues.The distribution and abundance of Propionibacterium showed differences between HCC and peritumoral tissues(P<0.05).In vitro studies demonstrated that Propionibacterium and its metabolite propionic acid(PA)inhibited the proliferation and migration of HCC cells(P<0.05).The expression of the proteins in NF-κB signaling pathway also decreased in HCC cells(P<0.05).CONCLUSION Microorganisms in HCC and normal liver tissues displayed significant disparities.The PA-producing bacterium Propionibacterium in HCC exerts an effect on the NF-κB pathway,thereby affecting the biological behavior of HCC.

关 键 词：Hepatocellular carcinoma Intratumoural microbiome 16S rRNA sequencing PROPIONIBACTERIUM 

分 类 号：R735.7[医药卫生—肿瘤]