丹参-葛根不同配伍比例对体外抗氧化活性的影响  

Antioxidant activities on different compatibility proportions of Salvia miltiorrhiza and Pueraria lobata

作　　者：张天怡 江川 施力 顾陈琳 包庭聿 颜扬鹏 庞汉青 ZHANG Tianyi;JIANG Chuan;SHI Li;GU Chenlin;BAO Tingyu;YAN Yangpeng;PANG Hanqing(Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease/College of Medicine(Institute of Translational Medicine),Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院(转化医学研究院)/江苏省中西医结合老年病防治重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2024年第4期36-41,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省高校自然科学基金面上项目(22KJB360018);扬州大学大学生科创基金资助项目(202311117019Z)。

摘　　要：为探究不同配伍比例的丹参与葛根体外抗氧化能力,采用水提法提取丹参葛根13个不同配伍比例(分别为1∶0、5∶1、3∶1、2∶1、3∶2、4∶3、1∶1、3∶4、2∶3、1∶2、1∶3、1∶5、0∶1)的有效成分,利用紫外分光光度法测定丹参葛根水提物的DPPH及ABTS自由基清除能力,以评估不同配伍比例的丹参葛根抗氧化活性。结果表明:丹参较葛根具有更强的抗氧化能力;与丹参、葛根单味药相比,丹参与葛根配伍比例为1∶2、3∶1和4∶3时,其水提物表现出更强的清除DPPH及ABTS自由基能力。综上,丹参与葛根配伍使用能增强其体外抗氧化活性,可为丹参葛根配伍应用提供一定的科学依据。This study was conducted to investigate the antioxidant effects of different compatibility proportions of Salvia miltiorrhiza and Pueraria lobata.The active ingredients of different proportions of Salvia miltiorrhiza and Pueraria lobata were extracted using the water extraction methods.Then the ultraviolet spectrophotometry was utilized to determine the scavenging ability of ABTS and DPPH free radical of various extracts of different proportions of S.miltiorrhiza and P.lobata.S.miltiorrhiza exhibited the stronger antioxidant activities than P.lobata;compared with the single herbs,when the proportions of S.miltiorrhiza and P.lobata were 1∶2,3∶1 and 4∶3,these samples possessed the strongest clearance of ABTS and DPPH free radical.S.miltiorrhiza and P.lobata could enhance the antioxidant capacities when decocting together.The study provides a solid scientific basis for the clinical application of S.miltiorrhiza and P.lobata.

关 键 词：丹参 葛根 配伍 水提法 抗氧化 

分 类 号：S853.74[农业科学—临床兽医学]