CD34和D2-40在原发性结肠腺癌中的表达及与伴淋巴结转移状态的关系  

Expression of CD34 and D2-40 in primary colon adenocarcinoma and association with concomitant lymph node metastatic status

作　　者：赵学影 侯仕璇 许远霞 陆进 ZHAO Xueying;HOU Shixuan;XU Yuanxia;LU Jin(Department of Anatomy,School of Basic Medicine,Bengbu Medical College Bengbu 233030,China;School of Clinical Medicine,Bengbu Medical College)

机构地区：[1]蚌埠医学院基础医学院人体解剖学教研室,安徽蚌埠233030 [2]蚌埠医学院临床医学院

出　　处：《包头医学院学报》2024年第10期19-22,50,共5页Journal of Baotou Medical College

基　　金：蚌埠医学院自然科学基金重点项目(2021byzd031);安徽省大学生创新训练项目(S202010367089,S202110367016)。

摘　　要：目的:探讨原发性结肠腺癌(colon adenocarcinoma,COAD)组织中血管和淋巴管标志物CD34(CD34 Molecule)和D2-40(又称Podoplanin)标记的癌胚抗原M2A的表达及其意义。方法:采用免疫组织化学Elivision TM plus法检测60例COAD组织和4例癌旁正常组织中CD34与D2-40标记的M2A蛋白表达,计数CD34阳性的微血管密度和D2-40阳性的微淋巴管密度,并分析其与COAD临床病理参数的关系。结果:在COAD组织中,CD34、D2-40阳性的微血管密度和微淋巴管密度高于癌旁正常组织(P<0.05)。COAD组织中的CD34微血管密度与病变部位有关,右半结肠癌患者中CD34微血管密度明显高于左半结肠癌患者(P<0.05);伴肠系膜淋巴结转移的COAD组织中CD34微血管密度与D2-40微淋巴管密度呈正相关(P<0.05)。结论:CD34和D2-40标记M2A蛋白在COAD组织中高表达,并且在伴肠系膜淋巴结转移状态下二者呈正相关,提示它们可能协同参与COAD淋巴结转移的发生。Objective:To investigate the expression of CD34 and D2-40-labeled carcinoembryonic antigen M2A in COAD tissues and its significance.Methods:The expression of CD34 and D2-40-labeled M2A protein was detected in 60 cases of COAD and 4 cases of adjacent normal tissues by immunohistochemical Elivision TM plus method,and the density of CD34-positive microvessels and D2-40-positive microlymphatic vessels were counted respectively,and its relationship with COAD clinicopathological parameters were analyzed.Results:In COAD tissues,microvessel density and microlymphatic vessel density of positive CD34 and D2-40 were significantly higher than those in adjacent normal tissues.The CD34 microvessel density in COAD tissue was related to the lesion site,the CD34 microvessel density in patients with right colon cancer was significantly higher than that in left colon cancer patients(P<0.05).CD34 microvessel density was positively correlated with D2-40 microvessel density in COAD tissues combining with mesenteric lymph node metastasis.Conclusion:CD34 and D2-40 labeled M2A protein are highly expressed in COAD tissues,and they are positively correlated with mesenteric lymph node metastasis,suggesting that they may be synergistically involved in the occurrence of COAD lymph node metastasis.

关 键 词：结肠腺癌 微血管密度 微淋巴管密度 CD34 D2-40 

分 类 号：R735.35[医药卫生—肿瘤]