阿加曲班联合线粒体移植对脑缺血再灌注损伤小鼠内皮功能及血流变学的影响  

Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheol⁃ogy in mice with cerebral ischemia⁃reperfusion injury

作　　者：陶颖俊 任腾竹 魏峰 刘新通 TAO Yingjun;REN Tengzhu;WEI Feng;LIU Xin-tong(Graduate Collaborative Training Base of the Second People's Hospital of Guangdong Province,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;Department of Neurology,the Second People's Hospital of Guangdong Province,University of South China Teaching Hospital,Guangzhou 510317,Guangdong,China;不详)

机构地区：[1]南华大学衡阳医学院、广东省第二人民医院研究生培养基地,湖南衡阳421001 [2]广东省第二人民医院神经内科,广东广州510317

出　　处：《实用医学杂志》2024年第19期2665-2671,共7页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2017A020215051)。

摘　　要：目的分析阿加曲班联合线粒体移植对脑缺血再灌注损伤小鼠内皮功能及血流变学的影响。方法50只SPF级KM小鼠,10只作为正常组,其余40只建模。38只小鼠建模成功,将其分为模型组9只、线粒体移植组9只、阿加曲班组10只、联合组10只。线粒体移植组给予外源性线粒体提取液,阿加曲班组给予阿加曲班,联合组给予阿加曲班联合线粒体移植,正常组、模型组注射生理盐水,干预7 d后观察小鼠脑梗死体积、脑组织病理形态、内皮功能、血流变学、凋亡相关蛋白相对表达量变化。结果与正常组相比,模型组、线粒体移植组、阿加曲班组、联合组脑梗死体积比、ET-1、全血黏度、血浆黏度水平、Caspase-3、Bax蛋白表达量上升,NO水平、Bcl-2蛋白表达量下降(P<0.05);与模型组相比,线粒体移植组、阿加曲班组、联合组脑梗死体积比、ET-1、全血黏度、血浆黏度水平、Caspase-3、Bax蛋白表达量下降,且联合组低于线粒体移植组、阿加曲班组,NO水平、Bcl-2蛋白表达量上升,且联合组高于线粒体移植组、阿加曲班组(P<0.05)。结论脑缺血再灌注损伤小鼠采用阿加曲班联合线粒体移植干预后内皮功能损伤改善,血流变学得到调节,脑损伤缓解。Objective To analyze the effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia-reperfusion injury.Methods 50 SPF grade Kunming mice,with 10 as the normal group,38 mices were successfully modeled and divided into a model group of 9,a mitochondrial transplantation group of 9,an Agatroban group of 10,and a combination group of 10.The mitochondrial transplantation group was given exogenous mitochondrial extract,the agatroban group was given agatroban,the combined group was given agatroban combined with mitochondrial transplantation,and the blank group and model group were injected with normal saline.Observation of changes in cerebral infarction volume,pathological morphology of brain tissue,endothelial function,hemorheology,and relative expression of apoptosis related proteins in mice after 7 days of intervention.Results Compared with normal group,cerebral infarction volume ratio,ET-1,whole blood viscosity,plasma viscosity,Caspase-3 and Bax protein expression levels were increased in model group,mitochondrial transplantation group,agatroban group and combined group,while NO level and Bcl-2 protein expression levels were decreased(P<0.05).Compared with the model group,the cerebral infarction volume ratio,ET-1,whole blood viscosity,plasma viscosity,Caspase-3 and Bax protein expressions in the mitochondrial transplantation group,agatroban group and combined group were decreased,and the combined group was lower than the mitochondrial transplantation group and agatroban group,and the NO level and Bcl-2 protein expression were increased.The combined group was higher than that of mitochondrial transplantation group and agatriban group(P<0.05).Conclusion In mice with cerebral ischemia-reperfusion injury,endothelial function injury was improved,hemorheology was regulated,and brain injury was alleviated after agatroban combined with mitochondrial transplantation.

关 键 词：阿加曲班 线粒体移植 脑缺血再灌注损伤 内皮功能 血流变学 

分 类 号：R745.1[医药卫生—神经病学与精神病学]