自拟丹星止颤方对帕金森病小鼠运动障碍、神经元损伤的改善作用及其机制  

Ameliorative effects of self-formulated Danxing Zhichan decoction on dyskinesia and neuronal damage in mice with Parkinson's disease and the mechanism

作　　者：胡楠 闫咏梅[1,2] 李涛 杨一帆[1,2] 彭涛 刘泽华 徐培丽 谢欣纯 HU Nan;YAN Yongmei;LI Tao;YANG Yifan;PENG Tao;LIU Zehua;XU Peili;XIE Xinchun(The First Clinical Medical College,Shaanxi University of Chinese Medicine,Xianyang 712000,China;不详)

机构地区：[1]陕西中医药大学第一临床医学院,陕西咸阳712000 [2]陕西中医药大学附属医院脑病一科 [3]陕西省人民医院中医科

出　　处：《山东医药》2024年第29期39-43,共5页Shandong Medical Journal

基　　金：国家岐黄学者支持项目[国中医药人教函(2022)6号];陕西省科技厅陕西省重点研发计划重点产业创新链(群)社会发展领域研究课题项目(2021ZDLSF04-13);陕西省中医脑病学重点实验项目(KF202316);中西医结合临床协作创新项目(GYZ-KJS-2021-001);陕西省教育厅重点实验室科研计划项目(19JS022);陕西省重点研发计划项目(2020ZDLSF05-14);研究生创新实践能力提升项目(CXSJ202325)。

摘　　要：目的观察自拟丹星止颤方对帕金森病小鼠运动障碍、神经元损伤的改善作用并探讨其机制。方法雄性C57BL/6小鼠48只,随机分为对照组、模型组、阳性对照组及丹星止颤方低、中、高剂量组,每组8只。除对照组外,各组均采用鱼藤酮溶液灌胃制备PD模型,对照组给予生理盐水灌胃,每天1次,连续28 d。造模成功后,丹星止颤方低、中、高剂量组给予丹星止颤方混悬液11.1、22.2、44.4 g/(kg·d)灌胃,阳性对照组给予美多芭混悬液112.5 mg/(kg·d)灌胃,对照组、模型组给予0.4 mL生理盐水灌胃,每天1次,连续28 d。采用网格实验及旋转杆实验观察小鼠运动能力,免疫组织化学法检测小鼠中脑黑质区α突触核蛋白(α-Syn),Western blotting法检测小鼠脑组织Toll样受体4(TLR4)、核因子κB(NF-κB)p65、α-Syn蛋白。结果小鼠移动潜伏期对照组<阳性对照组、丹星止颤方中剂量组<丹星止颤方高剂量组<丹星止颤方低剂量组、模型组,旋转杆保持时间对照组>阳性对照组、丹星止颤方中剂量组>丹星止颤方高剂量组>丹星止颤方低剂量组、模型组(P均<0.05)。小鼠中脑黑质区α-Syn阳性细胞比例对照组<阳性对照组、丹星止颤方中剂量组<丹星止颤方高剂量组<丹星止颤方低剂量组、模型组(P均<0.05)。小鼠脑组织TLR4、NF-κB p65、α-Syn蛋白表达对照组<阳性对照组、丹星止颤方中剂量组<丹星止颤方高剂量组<丹星止颤方低剂量组、模型组(P均<0.05)。结论自拟丹星止颤方可改善PD小鼠运动障碍及神经元损伤,其机制可能与调控TLR4/NF-κB通路从而抑制中枢神经炎症有关。Objective To observe the ameliorative effects of the self-formulated Danxing Zhichan decoction on the dyskinesia and neuronal damage in mice with Parkinson's disease and to explore the mechanism.Methods Forty-eight male C57BL/6 mice were randomly divided into the control group,model group,positive control group,and low-,medium-and high-dose Danxing Zhichan decoction groups,with eight mice in each group.The PD models were prepared by gavage with rotenone solution in all groups except the control group,and mice in the control group were given saline by gavage once a day for 28 d.After successful modeling,mice in the low-,medium-and high-dose Danxing Zhichan decoction groups were given Danxing Zhichan decoction 11.1,22.2 and 44.4 g/(kg·d)by gavage,and mice in the positive control group were given Medoba Suspension 112.5 mg/(kg·d)by gavage,and mice in the control group and model group were given 0.4 mL normal saline by gavage once a day for 28 d.The motor ability of mice was observed using the grid test and the rotating rod test,and theαsynaptic nuclear proteins(α-Syn)in the nigral region of the midbrain was detected by immunohistochemistry,and the Toll-like receptor 4(TLR4),nuclear factor-κB(NF-κB)p65,andα-Syn protein were detected by Western blotting.Results Mouse movement latency was as follows:control group<positive control group,medium-dose Danxing Zhichan decoction group<high-dose Danxing Zhichan decoction group<low-dose Danxing Zhichan decoction group and model group,and the rotating rod holding time was as follows:control group>positive control group,medium-dose Danxing Zhichan decoction group>high-dose Danxing Zhichan decoction group>low-dose Danxing Zhichan decoction group and model group(all P<0.05).The proportion of a-Syn positive cells in the substantia nigra region of mouse midbrain was as follows:control group<positive control group,medium-dose Danxing Zhichan decoction group<high-dose Danxing Zhichan decoction group<low-dose Danxing Zhichan decoction group and model group(all P<0.05),and the TL

关 键 词：帕金森病 丹星止颤方 运动障碍 神经元损伤 Toll样受体4、核因子κB 

分 类 号：R743.9[医药卫生—神经病学与精神病学]