喉癌肿瘤组织NOC2L、p53表达及其临床价值研究  

Expression of NOC2L and p53 in laryngeal carcinoma tumor tissue and its clinical value

作　　者：凡丹丹 高芳芳 邹婧 李鹏程 Fan Dandan;Gao Fangfang;Zou Jing;Li Pengcheng(Department of Otolaryngology,Wuhan Third Hospital,Hubei,Wuhan 430074,China;不详)

机构地区：[1]武汉大学附属同仁医院/武汉市第三医院耳鼻喉科,430074 [2]华中科技大学同济医学院附属协和医院肿瘤中心,430022

出　　处：《疑难病杂志》2024年第10期1177-1181,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：湖北省科技厅基金项目(2020CFA767)。

摘　　要：目的检测喉癌肿瘤组织中NOC2L、p53 mRNA表达并研究其临床价值。方法选取2020年1月—2022年1月武汉大学附属同仁医院诊治的喉癌患者103例(喉癌组)和喉良性疾病患者57例(对照组)。采用实时荧光定量聚合酶链反应检测组织NOC2L、p53 mRNA表达。比较喉癌不同临床病理特征中肿瘤组织NOC2L、p53 mRNA表达差异。受试者工作特征(ROC)曲线分析肿瘤组织NOC2L、p53表达预测喉癌患者预后不良的价值;多因素Cox回归模型分析喉癌预后不良的危险因素;Kaplan-Meier模型分析NOC2L、p53表达对喉癌生存期的影响。结果与对照组比较,喉癌组患者NOC2L表达升高,p53表达降低(t/P=19.307/<0.001、13.726/<0.001)。组织学分级3级、原发肿瘤大小≥3 cm、转移淋巴结数N2~3、远处转移M1及TNM分期Ⅲ~Ⅳ期喉癌患者肿瘤组织NOC2L mRNA表达高于组织学分级1~2级、原发肿瘤大小<3 cm、转移淋巴结数N0~1、远处转移M0及TNM分期Ⅰ~Ⅱ期患者(t/P=3.681/0.001、4.167/<0.001、2.554/0.012、4.005/<0.001、3.228/<0.001),p53 mRNA表达低于组织学分级1~2级、原发肿瘤大小<3 cm、转移淋巴结数N0~1、远处转移M0及TNM分期Ⅰ~Ⅱ期患者(t/P=4.162/<0.001、4.789/<0.001、4.053/<0.001、3.492/<0.001、3.724/<0.001);NOC2L、p53及二者联合预测喉癌预后不良的AUC分别为0.756、0.712、0.917,二者联合优于各自单独预测效能(Z=7.238、8.102,P<0.001)。NOC2L≥0.73、p53≤0.65、组织学分级3级、原发肿瘤大小≥3 cm、转移淋巴结数N2~3、远处转移M1及TNM分期Ⅲ~Ⅳ期为喉癌预后不良的独立危险因素[HR(95%CI)=5.328(1.455~9.201)、4.200(1.279~7.122)、1.992(1.127~2.857)、2.164(1.099~3.299)、2.228(1.304~3.152)、2.406(1.131~3.681)、2.514(1.278~3.762)];NOC2L≥0.81且p53≤0.54喉癌患者中位生存期显著低于NOC2L<0.81或p53>0.54患者中位生存期(Log Rankχ^(2)=9.033,P<0.001)。结论喉癌患者NOC2L及p53表达与病情严重程度及生存期显著相关,可为喉癌病情及�Objective To detect the NOC2L and p53 gene mRNA expression in laryngeal cancer tumor tissues and study their clinical value.Methods A total of 103 patients with laryngeal cancer(laryngeal cancer group)and 57 patients with benign laryngeal diseases(control group)from January 2020 to January 2022 were selected as the research subjects.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of NOC2L and p53 in tissues.The differences in the expression of NOC2L and p53 in tumor tissues of laryngeal carcinoma with different clinical pathological characteristics were compared.The ROC curve was used to analyze the sensitivity and specificity of NOC2L and p53 expression in predicting the poor prognosis of laryngeal cancer patients;the multivariate Cox regression model was used to analyze the risk factors for poor prognosis of laryngeal cancer;the Kaplan Meier model was used to analyze the effect of NOC2L and p53 expression on the survival of laryngeal cancer.Results Compared with the control group,NOC2L expression was increased and p53 expression was decreased in the laryngeal cancer group(t/P=19.307/<0.001,13.726/<0.001).NOC2L expression was higher in laryngeal cancer patients with histological grade 3,primary tumor diameter≥3 cm,number of metastatic lymph nodes N2~N3,distant metastasis M1 and TNM stageⅢ~IV than in patients with histological grade 1~2,primary tumor diameter<3 cm,number of metastatic lymph nodes N0~N1,distant metastasis M0 and TNM stage Ⅰ~Ⅱ(t/P=3.681/<0.001,4.167/<0.001,2.554/0.012,4.005/<0.001,3.228/<0.001),while p53 expression was lower in patients with histological grade 1~2,primary tumor diameter<3 cm,number of metastatic lymph nodes N0~N1,distant metastasis M0 and TNM stage I~II,number of metastatic lymph nodes N0~N1,distant metastasis M0 and TNM stage Ⅰ~Ⅱ patients(t/P=4.162/<0.001,4.789/<0.001,4.053/<0.001,3.492/<0.001,3.724/<0.001);the AUCs of NOC2L,p53 and their combination for predicting poor prognosis of laryngeal cancer were 0.756,0.712 and 0

关 键 词：喉癌 NOC2L P53 肿瘤组织 临床价值 

分 类 号：R739.65[医药卫生—肿瘤]