基于PI3K/Akt信号通路探讨脂联素对正畸牙移动及牙周组织改建的延缓作用  

Exploring the delaying effect of adiponectin on periodontal tissue remodelling under the PI3K/Akt signalling pathway

作　　者：蔡齐 蔚一博 任昊天 王彦蒽 陈玉 CAI Qi;WEI Yi-bo;REN Hao-tian;WANG Yan-en;CHEN Yu(Department of Stomatology,The First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学第一附属医院口腔科,上海200433

出　　处：《临床口腔医学杂志》2024年第9期538-542,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金委员会资助项目(项目编号:81800988)。

摘　　要：目的:基于PI3K/Akt信号通路探讨脂联素(adiponectin,APN)对牙周组织改建的延缓作用。方法:选择2020年1月~2023年1月本院收治正畸患者150例作为研究对象,收集患者性别、年龄、身体质量指数(body mass index,BMI)等基础信息,并采集牙周组织样本,检测牙周组织APN、破骨细胞分化因子(osteoclast differentiation factor,ODF)、核结合因子-α1(core binding factor-α1,CBF-α1)、磷脂酰肌醇-3-激酶(phosphatidyl-inositol-3-kinase,PI3K)、蛋白激酶B(protein kinase B,PKB/Akt)mRNA及蛋白表达水平;行正畸治疗4个月后,测量患者正畸牙移动距离。结果:不同性别患者APN、牙齿移动距离、ODF、CBF-α1、PI3K、Akt mRNA及蛋白表达水平差异无统计学意义(P>0.05);不同年龄、BMI患者APN、牙齿移动距离、ODF、CBF-α1、PI3K、Akt mRNA及蛋白表达水平差异有统计学意义(P<0.05)。Pearson相关分析结果显示,APN mRNA与牙齿移动距离、ODF mRNA、PI3K mRNA及Akt mRNA表达水平呈负相关(r=-0.72、-0.70、-0.76、-0.82,P<0.05),与CBF-α1 mRNA表达水平呈正相关(r=0.68,P<0.05);PI3K mRNA和Akt mRNA表达水平与牙齿移动距离、ODF mRNA表达水平呈正相关(r=0.78、0.76、0.81、0.81,P<0.05),与CBF-α1 mRNA表达水平呈负相关(r=-0.67、-0.69,P<0.05)。结论:正畸患者牙周组织改建会受到牙周组织APN表达水平的影响,其机制可能与APN调控PI3K/Akt信号通路相关蛋白的表达有关。Objective:To explore the delaying effect of adiponectin(APN)on periodontal tissue remodelling based on the PI3K/Akt signalling pathway.Methods:Totally 150 orthodontic patients admitted to our hospital from January 2020 to January 2023 were selected as the study subjects,patient gender,age,body mass index(BMI)and other basic information were recorded.And to collect periodontal tissue samples,APN,osteoclast differentiation factor(ODF),core binding factor-α1(CBF-α1),hosphatidyl-inositol-3-kinase(PI3K),protein kinase B(PKB/Akt)mRNA and protein expression levels were measured.After 4 months of orthodontic treatment,the tooth movement distance of the patient was measured.Results:The differences in APN,tooth movement distance,ODF,CBF-α1,PI3K,Akt mRNA and protein expression levels among patients of different genders were not statistically significant(P>0.05).The differences in APN,tooth movement distance,ODF,CBF-α1,PI3K,Akt mRNA and protein expression levels among patients of different ages and BMI were statistically significant(P<0.05).Pearson correlation analysis showed that APN mRNA was negatively correlated with tooth movement distance,ODF mRNA,PI3K mRNA,and Akt mRNA expression levels(r=-0.72,-0.70,-0.76,-0.82,P<0.05),and positively correlated with CBF-α1 mRNA expression level(r=0.68,P<0.05).PI3K mRNA and Akt mRNA expression levels were positively correlated with tooth movement distance and ODF mRNA expression levels(r=0.78,0.76,0.81,0.81,P<0.05)and negatively correlated with CBF-α1 mRNA expression levels(r=-0.67,-0.69,P<0.05).Conclusion:Periodontal tissue remodelling in orthodontic patients is affected by the expression level of APN in periodontal tissues,the mechanism of which may be related to the regulation by APN of the expression of proteins related to the PI3K/Akt signalling pathway.

关 键 词：正畸 牙周组织改建 脂联素 PI3K/AKT信号通路 

分 类 号：R783.5[医药卫生—口腔医学]