表达IBDV VP2基因的重组嵌合型新城疫病毒的免疫原性初步评价  

Immunogenicity Evaluation of Recombinant Chimeric Newcastle Disease Virus Expressing IBDV VP2 Gene

作　　者：周俏艳 杨宏春 陈浩 冯书风 李海珠 刘耕松 欧长波 ZHOU Qiaoyan;YANG Hongchun;CHEN Hao;FENG Shufeng;LI Haizhu;LIU Gengsong;OU Changbo(College of Animal Science and Technology,Guangxi University,Nanning,530004;Guangxi Engineering Research Center of Veterinary Biological Products,Nanning,530004;Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Control,Nanning,530004)

机构地区：[1]广西大学动物科学技术学院,南宁530004 [2]广西壮族自治区兽用生物制品工程研究中心,南宁530004 [3]广西畜禽繁育与疾病防控重点实验室,南宁530004

出　　处：《基因组学与应用生物学》2024年第8期1430-1438,共9页Genomics and Applied Biology

基　　金：广西科技基地和人才专项项目(桂科AD22035170)资助。

摘　　要：本研究为了研发能同时预防传染性法氏囊病病毒(infectious bursal disease virus,IBDV)和新城疫病毒(Newcastle disease virus,NDV)感染的二联苗,将IBDV VP2基因插入NDV LaSota疫苗株基因组的P基因和M基因之间,利用NDV反向遗传操作系统构建并成功获得重组病毒LS-IBDV-VP2。对重组病毒的鸡胚平均死亡时间(mean death time,MDT)、鸡胚半数感染量(egg infectious dose,EID50)和1日龄雏鸡脑内接种的致病指数(intracerebral pathogenicity index,ICPI)等生物学特性进行测定,并对18日龄SPF鸡胚进行免疫接种,利用血凝抑制实验(hemagglutination inhibition test,HI)测定NDV抗体以及间接酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)测定IBDV VP2抗体,评价重组病毒的免疫应答反应。结果显示,重组病毒LS-IBDV-VP2的EID50为108.5/mL、MDT为147 h、ICPI为0;NDV抗体效价在孵化后第14天达到最高,与LaSota疫苗株差异不显著(P>0.05),并在孵化后第14天也能产生较高水平的IBDV VP2抗体。本研究结果表明,重组病毒LS-IBDV-VP2与LaSota疫苗株具有相似的生物学特性,但重组病毒毒力更低,并且能够有效诱导机体产生抗体,具有成为预防IBDV和NDV二联候选疫苗株的潜力。In order to develop a vaccine that can simultaneously prevent infection by Infectious bursal disease virus(IBDV)and Newcastle disease virus(NDV),The IBDV VP2 gene was inserted between the P gene and the M gene of the NDV LaSota vaccine strain genome,and the recombinant virus LS-IBDV-VP2 was constructed and obtained successfully by using the NDV reverse genetic operating system.Mean death time(MDT),egg infectious dose(EID50)and intracerebral pathogenicity index of 1-day-old chicks inoculated with recombinant virus ICPI and other biological characteristics were determined,and the 18-day-old SPF chicken embryo was immunized,NDV antibody was determined by HI method and IBDV VP2 antibody was determined by indirect ELISA method,and the immune response of recombinant virus was evaluated.The results showed that the EID50 of LS-IBDV-VP2 was 108.5/mL,the MDT was 147 h and the ICPI was 0.The titer of NDV antibody reached the highest at 14 days after hatching,which was not significantly different from that of LaSota vaccine strain(P>0.05),and a higher level of IBDV VP2 antibody was also produced at 14 days after hatching.The results of this study indicate that the recombinant LS-IBDV-VP2 has similar biological characteristics to the LaSota vaccine strain,but its recombinant virus has lower virulence and can effectively induce the body to produce antibodies,which has the potential to be a candidate vaccine strain for the prevention of IBDV and NDV.

关 键 词：传染性法氏囊病病毒(IBDV) 新城疫病毒(NDV) VP2基因 反向遗传操作系统 鸡胚免疫 

分 类 号：S859.797[农业科学—临床兽医学]