富含sn-2位上α-亚麻酸的酶法酯交换二元油影响自发性高血压大鼠类固醇激素代谢的探索  

Exploration of enzyme-esterified oil withα-linolenic acid at the sn-2 position affecting spontaneously hypertensive rat steroid hormone metabolism

作　　者：侯佳乐 包音都古荣·金花[1,2,3] 迟源 马丽燕 刘玉滢 李雅雯 萨如拉 王越男[1] 呼格吉勒图[3] 侯荣伦 周欢敏 HOU Jiale;BAO YINDU GU RONG·Jinhua;CHI Yuan;MA Liyan;LIU Yuying;LI Yawen;SA Rula;WANG Yuenan;HUGE Jiletu;HOU Ronglun;ZHOU Huanmin(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010000,China;Inner Mongolia Key Laboratory of Biofabrication,Hohhot 010000,China;Inner Mongolia Hesige Green Industry Import and Export Co.Ltd.,Xilingol League 026321,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010010 [2]内蒙古生物制造重点实验室,内蒙古呼和浩特010010 [3]内蒙古贺斯格绿色产业进出口有限公司,内蒙古锡林郭勒盟026321

出　　处：《食品与发酵工业》2024年第19期33-39,共7页Food and Fermentation Industries

基　　金：内蒙古自治区自然科学基金项目(2023MS03034);内蒙古自治区科技计划项目(2023YFDZ0050,2021GG0348)。

摘　　要：该研究旨在探索富含sn-2位上α-亚麻酸的酶法酯交换二元油对自发性高血压大鼠(spontaneously hypertensive rat,SHR)类固醇激素分泌代谢的调控机理,为评价该二元油生理功效并提供数据基础。实验选用7周龄SHR,分为空白、二元油、羊尾油脂、亚麻籽油和菜籽油组,添加10%不同油脂饲料饲喂3个月。ELISA法测定血脂、肝激酶和性激素含量;q-PCR和Western-blot法检测类固醇激素相关基因和蛋白质表达量。结果显示,与空白组比较,二元油组大鼠血中低密度脂蛋白胆固醇降低、高密度脂蛋白胆固醇提高(P>0.05),肝激酶无显著性差异(P>0.05);睾酮水平高于菜籽油组,无显著性差异(P>0.05);二元油组和羊尾油脂组类固醇激素合成急性调节蛋白(synthesis acute regulatory protein,StAR)、胆固醇侧链裂解酶(CYP11A1)和细胞色素P45017A(CYP17A1)等基因和蛋白质表达显著高于菜籽油组(P<0.05)。由此,可以得出酶法酯交换二元油与其原料羊尾油脂相似,通过改善血脂和肝机能缓解SHR的病症,从而提高睾酮分泌,对其类固醇激素代谢具有有益影响。The objective of this study was to investigate the regulatory mechanism ofα-linolenic acid-rich enzyme-esterified oil on steroid hormone secretion and metabolism in spontaneously hypertensive rat(SHR),aiming to provide a data basis for evaluating the physiological efficacy of the enzyme-esterified oil.Seven-week-old SHR were divided into five groups:blank group,enzyme-esterified oil group,sheep tail fat group,flaxseed oil group,and rapeseed oil group.They were fed with 10%different oils for three months.The levels of blood lipids,liver kinase,and sex hormones were determined using ELISA;while the expression of steroid hormone-related genes and proteins was detected through q-PCR and Western-blot analysis.The results revealed that compared to the blank group,LDL-C levels decreased in the enzyme-esterified oil group while HDL-C levels increased(P>0.05).There was no significant difference observed in liver kinase activity among all groups(P>0.05).Although testosterone level in the esterified oil group was higher than that in the rapeseed oil group,there was no statistically significant difference(P>0.05).Notably,expression levels of steroid hormone synthesis acute regulatory protein(StAR),cholesterol side chain cleavage enzyme(CYP11A1),and cytochrome P45017A(CYP17A1)were significantly higher in both esterified oil and sheep tail fat groups compared to the rapeseed oil group(P<0.05).In conclusion,it can be inferred that enzyme-esterified oil is similar to its raw material sheep tail fat as it improves blood lipid profiles and liver function which subsequently enhances testosterone secretion along with its steroid hormone metabolism thereby alleviating symptoms associated with SHR.

关 键 词：酶法酯交换二元油 自发性高血压大鼠 肝机能 血脂 类固醇激素代谢 

分 类 号：TS201.4[轻工技术与工程—食品科学]