体外降糖降脂益生菌筛选及复配益生菌活性探究  

Screening of glucose-lowering and lipid-lowering probiotics in vitro and exploration of the activity of compound probiotics

作　　者：闫梦晴 郑彦懿 张璐 柳嘉 蓝彩娟 周晓莉 曾雪莹 张思璐 刘国玉 YAN Mengqing;ZHENG Yanyi;ZHANG Lu;LIU Jia;LAN Caijuan;ZHOU Xiaoli;ZENG Xueying;ZHANG Silu;LIU Guoyu(China National Research Institute of Food and Fermentation Industries Co.LTD.,Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases,Beijing 100015,China;Shenzhen Precision Health Food Technology Co.Ltd.,Shenzhen 518000,China)

机构地区：[1]中国食品发酵工业研究院有限公司,功能主食创制与慢病营养干预北京市重点实验室,北京100015 [2]深圳保时健生物工程有限公司,深圳518000

出　　处：《食品与发酵工业》2024年第19期56-62,共7页Food and Fermentation Industries

基　　金：中轻集团科技创新基金项目(ZQ2022YY02)。

摘　　要：为获得到降糖降脂综合能力较佳的复配益生菌,该研究以8种常用益生菌为研究对象,测定其体外降糖能力、胆固醇清除率、胃肠道耐受性及其他性能。以所测指标为因子,通过主成分分析方法筛选出综合能力较优的菌株,并进行菌株间拮抗分析及复配益生菌较佳比例的探究。此外,对较佳比例的复配益生菌进行益生元嗜好性分析。结果表明,植物乳杆菌CFFM1031和乳双歧杆菌CFFM2012降糖降脂综合能力较优,2种菌株之间无拮抗作用,与单一菌株或其他比例复配菌株相比,1∶1比例复配的菌株具有显著的胆固醇清除能力(43.56%),且复配益生菌在低聚半乳糖或水苏糖为碳源时增殖能力较强,表现出嗜好性。该研究可为益生菌协同增效研究及功能食品配方的开发提供参考。In order to obtain a compound probiotic with a better comprehensive ability to lower blood glucose and lipid levels,this study tested the effectiveness of eight commonly available probiotics in blood glucose lowering abilities,cholesterol clearance,gastrointestinal tolerance,and other activities in vitro.Principal component analysis was used to screen out strains with better comprehensive ability by taking the measured indicators as factors.Antagonism analysis was then performed among the strains,and the optimal proportion of compound probiotics was also determined.Additionally,a preference analysis for prebiotics was performed on the compound probiotic.The results showed that Lactobacillus plantarum CFFM1031 and Bifidobacterium lactis CFFM2012 were the most effective strains in reducing blood glucose and lipid levels.No antagonistic effect was observed between these two strains.The study found that a compound with a 1:1 ratio of L.plantarum CFFM1031 and B.lactis CFFM2012 significantly lowered cholesterol by 43.56%in vitro compared to single strains and other compound ratios.Moreover,the compound probiotic exhibited a higher proliferation ability when galacto-oligosaccharide and stachyose were used as carbon sources,indicating a preference for these sources.This study can provide a reference for probiotic synergism and the development of functional food formulations.

关 键 词：益生菌 降糖降脂能力 拮抗分析 复配益生菌 促增殖 

分 类 号：TS201.3[轻工技术与工程—食品科学]