亚甲蓝通过脑源性神经营养因子/原肌球蛋白相关激酶B通路减轻脑缺血再灌注大鼠的认知功能障碍  

Methylene blue ameliorates cognitive impairment in rats after cerebral ischemia-reperfusion via BDNF/TrkB pathway

作　　者：王伟[1,2] 黄永 方增焜[3] 覃国庆 李红波 蔡爱群 WANG Wei;HUANG Yong;FANG Zengkun;QIN Guoqing;LI Hongbo;CAI Aiqun(Department of Rehabilitation Medicine,International Zhuang Medical Hospital,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi Zhuang Autonomous Region 530201,China;Department of Rehabilitation Medicine,923 Hospital of the Chinese People's Liberation Army Joint Security Force,Nanning,Guangxi Zhuang Autonomous Region 530021,China;Department of Physiotherapy,Jiangbin Hospital,Guangxi Zhuang Autonomous Region,Nanning,Guangxi Zhuang Autonomous Region 530021,China;Department of Emergency Medicine,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi Zhuang Autonomous Region 530021,China)

机构地区：[1]广西中医药大学附属国际壮医医院康复医学科,广西壮族自治区南宁530201 [2]中国人民解放军联勤保障部队第九二三医院康复医学科,广西壮族自治区南宁530021 [3]广西壮族自治区江滨医院物理治疗科,广西壮族自治区南宁530021 [4]广西壮族自治区人民医院急诊科,广西壮族自治区南宁530021

出　　处：《安徽医药》2024年第11期2141-2145,I0001,共6页Anhui Medical and Pharmaceutical Journal

基　　金：广西壮族自治区中医药管理局计划课题(GXZYA20220204)。

摘　　要：目的研究亚甲蓝(MB)在缺血再灌注引起的认知功能障碍治疗中的作用及机制。方法2021年1月至2022年10月,以大脑中动脉栓塞(MCAO)大鼠为实验对象,MCAO术后立即腹腔注射亚甲蓝(10 mg/kg),运用改良大鼠神经功能缺损评分(mNSS)评估大鼠的神经损伤程度,水迷宫试验评估大鼠的认知能力,尼氏染色观测海马CA1区神经元结构,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡情况,蛋白质印迹法检测脑源性神经营养因子(BDNF)/原肌球蛋白相关激酶B(TrkB)蛋白表达水平。结果与缺血再灌注组相比,缺血再灌注后给予亚甲蓝的大鼠mNSS评分显著降低[24 h:(4.63±0.74)分;72 h:(3.5±1.07)分],认知能力显著提升[水迷宫潜伏期(14.58±2.90)s,穿越平台次数(7.25±1.67)次],梗死体积显著减少[(30.54±5.11)mm^(3)],海马CA1区神经元结构损伤减轻,神经元凋亡数量显著下降[(6.12±2.03)个],BDNF/TrkB蛋白表达量显著增加(BDNF:0.53±0.01;TrkB:0.65±0.08)。结论亚甲蓝可减轻脑缺血再灌注大鼠的认知功能障碍,且可能系通过BDNF/TrkB通路发挥了作用。Objective To analyze the therapeutic effect of Methylene blue and its mechanism in cognitive impairment after cerebral ischemia-reperfusion.Methods Rats with MCAO surgery were used as experiment subjects from January 2021 to October 2022.mNSS was used to evaluate the neurological damage of rats.Morris water maze test was used to evaluate cognitive ability in rats.Nissl staining was used to detect neuronal structure in hippocampus CA1 zone in rats.TUNEL was used to detect cell apoptosis.Protein expression of BDNF/TrkB was detected by Western Blotting.Results Compared to the ischemia-reperfusion group,rats administered methylene blue after ischemia-reperfusion exhibited significantly reduced mNSS scores[24 h:(4.63±0.74)points;72 h:(3.5±1.07)points,enhanced cognitive abilities[Morris water maze latency(14.58±2.90)s,crossover platform count 7.25±1.67],decreased infarct volume[(30.54±5.11)mm^(2)],alleviated neuronal structural damage in the hippocampal CA1 region,significantly lower numbers of apoptotic neurons(6.12±2.03),and a notable increase in BDNF/TrkB protein expression levels(BDNF:0.53±0.01;TrkB:0.65±0.08).Conclusion Methylene blue ameliorates cognitive impairment in rats after cerebral ischemia-reperfusion via BDNF/TrkB pathway.

关 键 词：亚甲蓝 再灌注损伤 认知能力 脑源性神经营养因子/原肌球蛋白相关激酶B通路 

分 类 号：R743[医药卫生—神经病学与精神病学]