血清微RNA-183-5p和微RNA-144-3p水平与脑出血病人脑出血量及认知功能的相关性  

Correlation between serum miRNA-183-5p and miRNA-144-3p levels with cerebral hemorrhage volume and cognitive function in patients with cerebral hemorrhage

作　　者：朱少良[1] 涂强[1] 汪华[1] ZHU Shaoliang;TU Qiang;WANG Hua(Department of Neurology,Jingzhou First People's Hospital,Jingzhou,Hubei 434000,China)

机构地区：[1]荆州市第一人民医院神经内科,湖北荆州434000

出　　处：《安徽医药》2024年第11期2235-2239,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的检测脑出血病人血清中微RNA(miRNA)-183-5p和miRNA-144-3p表达水平,分析两者与脑出血量及认知功能的相关性。方法回顾性分析2020年8月至2021年8月荆州市第一人民医院收治的符合纳入、排除标准的89例脑出血病人的临床资料(观察组),另收集体检健康者89例为对照组。根据头颅影像学检查结果,将观察组分为少量出血组(<15 mL)31例,中量出血组(15~30 mL)36例和大量出血组(>30 mL)22例。采用实时荧光定量聚合酶链式反应(qRT-PCR)检测血清miRNA-183-5p和miRNA-144-3p表达水平,Pearson相关分析脑出血量与血清miRNA-183-5p、miRNA-144-3p表达水平的相关性,根据是否出现认知功能障碍,将观察组病人分为预后良好组57例和预后不良组32例;受试者操作特征曲线(ROC曲线)分析血清miRNA-183-5p和miRNA-144-3p水平对脑出血病人预后的评估价值。结果观察组血清miRNA-183-5p表达水平0.67±0.16低于对照组1.01±0.24,血清miRNA-144-3p的表达水平3.53±0.73高于对照组1.05±0.21(P<0.05)。大、中、少出血组血清miRNA-183-5p水平为0.49±0.11、0.69±0.11、0.78±0.21,依次显著降低(P<0.05):大量出血组血清miRNA-144-3p水平3.86±0.75与中量出血组水平3.58±0.68显著高于少量出血组水平3.17±0.65(P<0.05)。脑出血量与血清miRNA-183-5p表达呈负相关(r=−0.51,P<0.05)、与miRNA-144-3p表达呈正相关(r=0.54,P<0.05)。预后不良组血清miRNA-183-5p水平0.50±0.12低于预后良好组0.77±0.18,血清miRNA-144-3p水平3.75±0.62高于预后良好组3.41±0.34(P<0.05)。血清miRNA-183-5p、miRNA-144-3p单独及联合评估病人预后的AUC分别是0.94、0.79和0.96,二者联合检测的AUC、灵敏度高于各自单独检测。结论脑出血病人血清miRNA-183-5p水平降低,miRNA-144-3p水平升高,与脑出血量相关性较强,二者联合可评估病人认知功能障碍的发生。Objective To detect the expression levels of miRNA-183-5p and miRNA-144-3p in serum of patients with cerebral hemorrhage,and to analyze the correlation between the two with the amount of cerebral hemorrhage and cognitive function.Methods The clinical data of 89 patients with intracerebral hemorrhage who met the inclusion and exclusion criteria admitted to Jingzhou First People's Hospital from August 2020 to August 2021 were retrospectively analyzed(observation group).Another 89 healthy subjects'clinical data who underwent physical examination were collected as the control group at the same time.According to the results of skull imaging,the observation group was divided into different subgroups:31 cases in the small amount of bleeding group(<15 mL),36 cases in the medium amount of bleeding group(15-30 mL)and 22 cases in the large amount of bleeding group(>30 mL).The expression levels of miRNA-183-5p and miRNA-144-3p in serum of patients were detected by quantitative real-time polymerase chain reaction(qRTPCR),Pearson correlation was applied to analyze the correlation between the amount of cerebral hemorrhage and the expression levels of miRNA-183-5p and miRNA-144-3p in serum,the patients were grouped into a good prognosis group(n=57)and a poor prognosis group(n=32)according to whether there was cognitive impairment or not;receiver operating characteristic(ROC)curve was applied to analyze the appraisal value of serum miRNA-183-5p and miRNA-144-3p levels in patients with intracerebral hemorrhage.Results The serum miRNA-183-5p expression level in the observation group 0.67±0.16 was lower than that in the control group 1.01±0.24,and the serum miRNA-144-3p expression level 3.53±0.73 was higher than that in the control group 1.05±0.21(P<0.05).The level of serum miRNA-183-5p in the groups with different bleeding volume was that in the group with large bleeding volume,medium bleeding volume and small bleeding volume were 0.49±0.11,0.69±0.11,0.78±0.21(P<0.05).The level of serum miRNA-144-3p in the groups with large

关 键 词：脑出血 微RNA-183-5p 微RNA-144-3p 脑出血量 预后 

分 类 号：R743.34[医药卫生—神经病学与精神病学]