天然橡胶中蛋白质的酰胺Ⅲ带二级结构及其热变性  

Secondary structures of protein amideⅢband and its thermal denaturation in natural rubber

作　　者：雷晓静 王春润 张美环 刘荣 常明[2] 张勇[1] 于宏伟[2] 徐元媛 LEI Xiao-jing;WANG Chun-run;ZHANG Mei-huan;LIU Rong;CHANG Ming;ZHANG Yong;YU Hong-wei;XU Yuan-yuan(School of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;College of Chemical Engineering,Shijiazhuang University,Shijiazhuang 050035,China)

机构地区：[1]河北科技大学化学与制药工程学院,石家庄050018 [2]石家庄学院化工学院,石家庄050035

出　　处：《合成橡胶工业》2024年第5期389-393,共5页China Synthetic Rubber Industry

基　　金：河北省高等学校科学技术研究项目(ZC2021250);石家庄市高等教育科学研究项目(20241073);石家庄学院教学改革研究与实践项目(JGXM-202408 Y);石家庄学院大学生创新创业训练计划项目(SCXM 0289)。

摘　　要：采用中红外(MIR)光谱对天然橡胶中蛋白质酰胺Ⅲ带的二级结构及其热变性进行了研究。变温去卷积MIR光谱结果表明,酰胺Ⅲ带二级结构在293~393 K的温度范围内升温时,无规则卷曲、β-折叠、α-螺旋及β-转角二级结构的红外吸收峰强度降低。二维MIR光谱结果表明,酰胺Ⅲ带二级结构对热的敏感程度存在较大差异,其中无规则卷曲二级结构最先改变。热扰动因素下,无规则卷曲二级结构改变是整个酰胺Ⅲ带二级结构改变的关键步骤。Secondary structures of protein amideⅢband and its thermal denaturation in natural rubber were studied by midinfrared(MIR)spectroscopy.The result of variable temperaturet deconvolution MIR spectroscopy showed that the infrared absorption peak intensity of irregular curled,β-folded,α-spiral andβ-corner se-condary structures decreased when the temperature increased from 293 to 393 K.The result of two-dimensional MIR spectroscopy showed that there were significant differences in the sensitivity of amideⅢband secondary structures to heat,with the irregular curled secondary structure being the first to change.Under thermal disturbance factor,the irregular curled secondary structure change was a key step in the overall secondary structure change of the amideⅢband.

关 键 词：天然橡胶 蛋白质二级结构 酰胺Ⅲ带 二维中红外光谱 热变性 

分 类 号：TQ330.73[化学工程—橡胶工业]