基于SIDA-GC-QqQ-MS/MS的烤烟中痕量香气化合物2-乙酰基-1-吡咯啉的测定  

Determination of trace aroma compound 2-acetyl-1-pyrroline in flue-cured tobacco based on SIDA-GC-QqQ-MS/MS

作　　者：李舒畅 李军[3] 张新龙[3] 常诗瑜 庞雪莉[1] 孔凡玉[1] LI Shuchang;LI Jun;ZHANG Xinlong;CHANG Shiyu;PANG Xueli;KONG Fanyu(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266101,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;China Tobacco Shandong Industry Co.,Ltd.,Jinan 250014,China)

机构地区：[1]中国农业科学院烟草研究所,青岛市266101 [2]中国农业科学院研究生院,北京市100081 [3]山东中烟工业有限责任公司,济南市250014

出　　处：《中国烟草学报》2024年第5期30-38,共9页Acta Tabacaria Sinica

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC07)。

摘　　要：【目的】精确测定烤烟中痕量香气化合物2-乙酰基-1-吡咯啉(2AP)的含量。【方法】采用稳定同位素稀释结合气相色谱-串联三重四级杆质谱仪MRM多反应监测模式对烤烟中2-乙酰基-1-吡咯啉进行精确定量分析。【结果】(1)方法在2~200μg/kg范围内线性良好(R^(2)=0.9998),检测限为0.38μg/kg,定量限为1.26μg/kg,平均回收率为90.75%~96.10%。(2)烤烟烟叶中2AP含量为2.02~62.48μg/kg,主流烟气中含量为7.29~94.12μg/kg,其中粒相物和气相物的含量范围分别为2.08~79.08μg/kg和5.15~18.68μg/kg。【结论】方法稳定可行、高效简便,具有较高的精度和灵敏度,可为烟用香精香料配方设计开发提供数据支撑,为烤烟中其他痕量香气化合物的精确定量分析研究提供方法参考。[Objective]This study aims to accurately determine the content of trace aroma compound 2-acetyl-1-pyrroline(2AP)in flue-cured tobacco.[Methods]A stable isotope dilution analysis-gas chromatography-triple quadrupole mass spectrometry(SIDA-GC-QqQ-MS/MS MRM)method in multiple reaction monitoring(MRM)mode was used for accurate quantitative analysis of 2-acetyl-1-pyrroline in flue-cured tobacco.[Results]The method showed good linearity in the range of 2μg/kg to 200μg/kg with R^(2)=0.9998,with a detection limit of 0.38μg/kg and a quantification limit of 1.26μg/kg,,and an average recovery rate of 90.75%~96.10%.The content of 2AP in flue-cured tobacco leaves ranged from 2.02μg/kg to 62.48μg/kg,and that in mainstream smoke was 7.29μg/kg to 94.12μg/kg,with the content in particulate and gaseous phases ranging from 2.08μg/kg to 79.08μg/kg and 5.15μg/kg to 18.68μg/kg,respectively.The sensory intensity of“glutinous rice”-like aroma is positively correlated with the 2AP content in mainstream smoke.[Conclusion]This proposed method is stable,feasible,efficient and simple,with high accuracy and sensitivity.It can provide data support for the design and development of tobacco flavor and fragrance formulations and offer a methodological reference for the precise quantitative analysis of other trace aroma compounds in flue-cured tobacco.

关 键 词：烤烟烟叶 主流烟气 2-乙酰基-1-吡咯啉 气相色谱-串联三重四级杆质谱(GC-QqQ-MS/MS) 稳定同位素稀释定量(SIDA) 

分 类 号：TS411[农业科学—烟草工业] O657.63[理学—分析化学]