miR-142-3p和miR-598-3p在卵巢良恶性肿瘤组织中的表达及其临床意义  

Expression and clinical significance of miR-142-3p and miR-598-3p in ovarian benign and malignant tumor tissues

作　　者：汪莹[1] 姚满红 刘玉凤[1] 艾志刚[1] WANG Ying;YAO Manhong;LIU Yufeng;AI Zhigang(Department of Gynecology,Tangshan Maternal and Child Health Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院妇科,河北唐山063000

出　　处：《中国优生与遗传杂志》2024年第7期1399-1404,共6页Chinese Journal of Birth Health & Heredity

基　　金：河北省卫生健康委医学科学研究课题计划项目(20191526)。

摘　　要：目的 探讨卵巢上皮癌(EOC)和卵巢良性肿瘤组织中微小RNA(miR)-142-3p和miR-598-3p的表达,以及对EOC鉴别诊断的临床意义。方法 选取2021年1月至2023年5月期间在唐山市妇幼保健院接受手术治疗的EOC或良性卵巢肿瘤患者作为研究对象,先在训练集组织样本中进行miRNA测序和癌症基因组图谱(TCGA)分析筛选出具有差异表达的miRNA,进一步在验证集组织中进行验证,使用定量反转录聚合酶链反应测定miRNAs的表达情况,并用受试者操作特征(ROC)曲线分析组织miRNA鉴别卵巢良恶性肿瘤的能力。结果 在训练集的组织样本中发现33个差异表达miRNA(P<0.01)。经TCGA数据分析,miR-142-3p、miR-598-3p、miR-181b-5p、miR-19a-3p、miR-21-3p和miR-55b-3p在肿瘤和邻近正常组织之间也有差异表达。在验证集样本中证实,EOC组织中miR-142-3p和miR-598-3p水平低于卵巢良性肿瘤组(P<0.05)。EOC组织中miR-142-3p(P=0.001)和miR-598-3p(P=0.013)低表达与更严重的FIGO分期有关。ROC曲线显示miR-142-3p、miR-598-3p鉴别诊断EOC的曲线下面积(AUC)分别为0.629和0.695,联合诊断更是具有协同作用(AUC=0.802)。另外,2-miRNA组合(miR-142-3p+miR-598-3p)对于传统的糖类抗原125和人附睾蛋白4血清学肿瘤标志物的鉴别诊断AUC产生了增效作用(AUC=0.862)。结论 组织miR-142-3p和miR-598-3p可能是一个有用的生物标志物,可以增强血清CA125、HE4区分EOC和卵巢良性肿瘤的价值。Objective To investigate the expression of microRNAs(miR)-142-3p and miR-598-3p in ovarian epithe-lial carcinoma(EOC)and benign ovarian tumor tissues,and the clinical significance of miR-142-3p and miR-598-3p in the differential diagnosis of EOC.Methods Patients with EOC or benign ovarian tumors who underwent surgery in Tangshan Maternal and Child Health Hospital from January 2021 to May 2023 were selected as the research objects,and miRNA se-quencing and cancer genome atlas(TCGA)analysis were performed in the training set tissue samples to screen out the differ-entially expressed miRNAs,and further verified in the validation set tissues,the expression of miRNAs was determined by quantitative reverse transcription polymerase chain reaction(qRT-PCR),and the ability of tissue miRNAs to identify benign and malignant ovarian tumors was analyzed by the receiver operator characteristic(ROC)curve.Results In training set,33 miRNAs were differentially expressed between the two groups(P<0.01).miR-142-3p,miR-598-3p,miR-181b-5p,miR-19a-3p,miR-21-3p,and miR-55b-3p were also differentially expressed between tumors and adjacent normal tissues in TCGA ovarian cancer samples.In validation set,it was found that the levels of miR-142-3p and miR-598-3p in EOC tissues were lower than those in patients with benign ovarian tumors(P<0.05).Low expression of miR-142-3p(P=0.001)and miR-598-3p(P=0.013)in EOC tissues was associated with more severe FIGO stage.The ROC curves showed that the area under the curve(AUC)of miR-142-3p and miR-598-3p in the differential diagnosis of EOC were 0.629 and 0.695,respectively.There is a synergistic effect in the combined diagnosis of miR-142-3p and miR-598-3p(AUC=0.802).In addition,the 2-miRNA combination(miR-142-3p+miR-598-3p)had a synergistic effect on the differential diagnosis AUC of traditional carbohydrate antigen 125 and human epididymal protein 4 serological tumor markers(AUC=0.862).Conclusion Tissue miR-142-3p and miR-598-3p may be useful biomarkers that can enhance the value of serum CA125 and H

关 键 词：卵巢上皮癌 miR-142-3p miR-598-3p 

分 类 号：R737.31[医药卫生—肿瘤]