白背叶楤木芽苞乙酸乙酯部位中芳香族化合物及其体外抗炎活性  

Aromatic compounds from ethyl acetate fraction of Aralia chinensis var.nuda and their anti-inflammation activities in vitro

作　　者：陈帅 陈邦 陈宇凡 陈福长 钱海珊 何红平 李宝晶 CHEN Shuai;CHEN Bang;CHEN Yu-fan;CHEN Fu-chang;QIAN Hai-shan;HE Hong-ping;LI Bao-jing(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500;The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500;Yunnan Key Laboratory of Sustainable Devolopment and Utilization of Southern Medicine,Yunnan University of Chinese Medicine,Kunming 650500)

机构地区：[1]云南中医药大学中药学院,昆明650500 [2]云南中医药大学第一附属医院,昆明650500 [3]云南中医药大学云南省南药可持续利用研究重点实验室,昆明650500

出　　处：《中南药学》2024年第10期2596-2601,共6页Central South Pharmacy

基　　金：国家自然科学基金(No.82260832,No.81860760);云南省中医联合专项(No.202101AZ070001-159);云南省重大科技专项(No.202002AA100007)。

摘　　要：目的研究白背叶楤木Aralia chinensis var.nuda芽苞乙醇提取物乙酸乙酯部位芳香族化合物及其体外抗炎活性。方法白背叶楤木芽苞70%乙醇提取物乙酸乙酯部位浸膏采用硅胶柱色谱分离法、RP-18反相色谱分离法、薄层色谱法、凝胶过滤层析法、半制备高效液相色谱法分离。根据理化性质及核磁共振波谱数据鉴定化合物结构。使用Griess法测定化合物对脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7产生一氧化氮(NO)的抑制活性。结果从中分离得到14个芳香族化合物,分别鉴定为松脂素(1)、3'-O-去甲基表松脂素(2)、(+)-表松脂醇(3)、绿原酸甲酯(4)、绿原酸乙酯(5)、3,4-二咖啡酰基奎宁酸甲酯(6)、1-O-caffeoylglycerol(7)、drypetesin B(8)、papuline(9)、高山黄芩素(10)、红花素(11)、trichocarpine(12)、2-(3',4'-二羟苯基)-1,3-胡椒环-5-醛(13)、4-methoxy-1,2-dihydrocyclobutabenzene(14)。体外抗炎活性研究结果表明,化合物13对LPS诱导的RAW264.7细胞NO释放有较强抑制活性,在50μmol·L^(-1)浓度下,NO生成抑制率为(59.28±1.24)%。结论化合物1~2、4~14为首次从楤木属植物中分离得到,化合物1~14为首次从白背叶楤木中分离得到,化合物13具有良好的体外抗炎活性。Objective To determine the aromatic compounds and anti-inflammation activities in vitro of ethanol extract of the bud of Aralia chinensis var.nuda.Methods The 70%ethanol extract from the bud of Aralia chinensis var.nuda was separated by silica gel column chromatography,RP-18 reversed-phase chromatography,thin-layer chromatography,gel filtration chromatography and semi-preparative high performance liquid chromatography.According to the physical and chemical properties and NMR data,the structures of the compounds were identified.The inhibition activities of the compounds on NO production in LPS-induced mouse macrophages RAW264.7 cells were evaluated by Griess assay.Results Totally 14 aromatic compounds were isolated and identified as rosin(1),3'-O-demethylepipinoresin(2),(+)-epiterpineol(3),methyl chlorogenic acid(4),ethyl chlorogenic acid(5),3,4-diccaffeoyl quinic acid methyl ester(6),1-O-caffeoylglycorol(7),drypetesin B(8),papuline(9),baicalein(10),carthamin(11),trichocarpine(12),2-(3',4'-dihydroxyphenyl)-1,3-piperonal-5-aldehyde(13),and 4-methoxy-1,2-dihydrocyclobutanzene(14).Compounds 13 exhibited strong inhibition on LPS-induced RAW264.7 cells,with the inhibition rates of NO production at(59.28±1.24)%at the concentration of 50μmol·L^(-1).Conclusion Compounds 1~2,4~14 have been isolated from this genus Aralia for the first time,and compounds 1~14 from Aralia chinensis var.nuda for the first time.Compound 13 exhibits great anti-inflammation activities in vitro.

关 键 词：白背叶楤木 芳香族化合物 分离鉴定 抗炎活性 

分 类 号：R914[医药卫生—药物化学] R285[医药卫生—药学]