复合菌种发酵人参提取物对小鼠毛囊细胞增殖作用的研究  

Effect of ginseng compound fermented extract on the proliferation of mouse hair follicle cells

作　　者：王井侠 李梦雪 张雅楠 王明帅 许庆瑞 WANG Jing-xia;LI Meng-xue;ZHANG Ya-nan;WANG Ming-shuai;XU Qing-rui(Heilongjiang Academy of Chinese Medical Sciences,Harbin 150000)

机构地区：[1]黑龙江省中医药科学院,哈尔滨150000

出　　处：《中南药学》2024年第10期2674-2679,共6页Central South Pharmacy

基　　金：黑龙江省中医药科研项目(No.ZHY2023-040)。

摘　　要：目的评价复合菌种发酵人参提取物对小鼠毛囊细胞的增殖效果,预测对小鼠毛囊细胞有增殖作用的有效成分,探究复合发酵提取物优势的物质基础。方法采用枯草芽孢杆菌、鼠李糖乳杆菌、干酪乳杆菌三种菌种复合发酵人参,经提取后获得复合菌种发酵人参提取物,通过CCK-8法检测小鼠毛囊细胞的增殖率,评价复合菌种发酵人参提取物对小鼠毛囊细胞的增殖作用;通过超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS)联用技术,分析人参发酵前后的差异性化学成分。结果复合菌种发酵人参提取物对小鼠毛囊细胞增殖率为59.83%,增殖效果优于未发酵人参提取物;UPLC-Q-TOF-MS分析结果显示,复合菌种发酵人参提取物与未发酵人参提取物成分相比较共有26个差异性成分,其中13个成分在发酵后含量升高,13个成分含量下降。结论复合菌种发酵人参提取物促进小鼠毛囊细胞增殖有较大优势,人参经复合发酵后,豆甾醇、三叶豆苷、人参酚、人参皂苷Rh2、人参皂苷Rh4等13个成分含量升高,推测其可能是对小鼠毛囊细胞有增殖作用的有效成分。Objective To evaluate the proliferative effect of ginseng compound fermented extract on mouse hair follicle cells,predict the effective proliferation promoting components in these cells,and determine the material basis for related mechanism.Methods The method involved co-fermentation of ginseng with Bacillus subtilis,Lactobacillus casei,and Lactobacillus acidophilus.After the extraction,ginseng fermented extract was obtained.The proliferation rate of mouse hair follicle cells was assessed by CCK-8 assay to evaluate the proliferative effect of ginseng compound fermented extract.Ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze the differential chemical components before and after the ginseng fermentation.Results The ginseng compound fermented extract exhibited a proliferation rate of 59.83%on mouse hair follicle cells,surpassing the effect of non-fermented ginseng extract.UPLC-Q-TOF-MS analysis revealed 26 differential components between the ginseng compound fermented extract and ginseng extract:13 components increasing and 13 decreasing after the fermentation.Conclusion Ginseng compound fermented extract demonstrates great advantages in promoting the proliferation of mouse hair follicle cell.After the fermentation,the content of 13 components,including soyasaponin,daidzein glucoside,ginsenoside Rb2,ginsenoside Rh2,and ginsenoside Rh4 are increased,suggesting their potential as effective components in promoting mouse hair follicle cell proliferation.

关 键 词：人参 发酵 毛囊 UPLC-Q-TOF-MS 

分 类 号：R285.5[医药卫生—中药学]