两种方法建立肝癌皮下瘤块模型的比较  

Comparison of the Embellishment of Subcutaneous Tumor Model of Hepatocellular Carcinoma with Two Methods

作　　者：高洪飞 Gao Hongfei(Department of Interventional Medicine,Linyi Central Hospital,Linyi,Shandong,276400,China)

机构地区：[1]临沂市中心医院介入科,山东临沂276400

出　　处：《黑龙江医学》2024年第20期2486-2488,共3页Heilongjiang Medical Journal

摘　　要：目的:采用注射Walker-256肿瘤细胞的方法建立Wistar鼠和BALB/c裸鼠皮下瘤块模型。方法:抽取含Walker-256肿瘤细胞的BALB/c裸鼠腹水,进行离心洗涤,将洗涤液分别接种于Wistar鼠及BALB/c裸鼠腹股沟区皮下形成皮下瘤块。观察并比较两种不同实验鼠所建立的皮下瘤块模型成瘤率及瘤体生长速度。实验结束后,采用免疫组织化学法检测皮下瘤的微血管密度(MVD)及血管内皮生长因子(VEGF)的表达。结果:两种方法成瘤率均为100%,但是裸鼠皮下瘤块生长速度快于Wistar鼠皮下瘤;裸鼠组的MVD与VEGF表达均高于Wistar组,差异有统计学意义(t=8.52、5.13,P<0.05)。结论:用Wistar鼠或裸鼠作为肝癌皮下瘤块模型均可以满足临床试验研究的需要,但由于裸鼠为免疫缺陷动物,瘤块生长速度会明显快于Wistar鼠,且肿瘤血管生成指标更高,故选择肝癌瘤块生长模型时,裸鼠为最佳选择。Objective:To establish subcutaneous tumor mass model of Wistar and BALB/c nude mice by injecting Walker-256 tumor cells.Methods:The ascites of BALB/c nude mice containing Walker-256 tumor cells were extracted,centrifugally washed,and the washing solution was inoculated into the subcutaneous inguinal region of Wistar mice and BALB/c nude mice to form subcutaneous tumor masses.The tumor formation rate and tumor growth rate of the subcutaneous tumor mass model were observed and compared between two different experimental mice.After experiment,the microvascular density(MVD)and the expression of vascular endothelial growth factor(VEGF)were detected by immunohistochemistry.Results:Success rate of both methods were 100%.The growth rate of tumor mass in BALB/c nude mice was faster than that in Wistar rats.And the molecular expression of VEGF and MVD in the group of BALB/c nude mice were both higher than those of Wistar rats(t=8.52,5.13;P<0.05).Conclusion:Both Wistar mice and nude mice can meet the needs of clinical trials and studies.However,as nude mice are immunodeficient animals,the growth rate of tumor mass is significantly faster than that of Wistar mice,and the tumor angiogenesis index is higher.Therefore,nude mice are the best choice when choosing the growth model of liver cancer tumor mass.

关 键 词：瘤块模型 Walker-256肿瘤细胞 WISTAR大鼠 BALB/C裸鼠 微血管密度 血管内皮生长因子 

分 类 号：R73-354[医药卫生—肿瘤]