宁夏地区2017—2023流感监测年乙型流感病毒血凝素基因变异状况及序列分析  

Mutation and sequence analysis of influenza B virus hemagglutinin gene in Ningxia Hui Autonomous Region from 2017 to 2023

作　　者：孙晓强[1] 穆婷 袁芳[1] 李龙山 张薇 马学平[1] SUN Xiao-qiang;MU Ting;YUAN Fang;LI Long-shan;ZHANG Wei;MA Xue-ping(Ningxia Hui Autonomous Region Center for Disease Control and Prevention,Ningxia Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区疾病预防控制中心,宁夏银川750004

出　　处：《江苏预防医学》2024年第4期418-421,449,共5页Jiangsu Journal of Preventive Medicine

基　　金：宁夏回族自治区青年拔尖人才培养工程项目(RQ0049)。

摘　　要：目的了解近年宁夏地区乙型流感病毒的病原学检测情况和血凝素(hemagglutinin,HA)基因变异情况。方法采用时空分布抽样方法选取2017—2023年自治区疾控中心分离的乙型流感毒株44株,提取RNA,通过RT-PCR反应扩增HA片段并测序,测序结果利用生物信息软件进行分析。结果对分离的44株乙型流感分离株进行全基因组测序分析,对比疫苗株B/California/12/2015,15株BY系中有7个毒株发生抗原位点改变,涉及120环抗原表位的R136G和L137H的改变,均涉及受体结合位点改变;对比疫苗株B/Colorado/06/2017,29株BV系毒株有26株发生了抗原位点变异,涉及4个抗原表位的11个受体结合位点。涉及受体结合位点2个的3个位点发生改变;未检出潜在糖基化位点发生改变。本次研究中的所有BY系的毒株均隶属于Clade 3分支,所有BV系的毒株全部属于Clade 1A分支。结论2017—2023流感监测年,乙型流感在宁夏地区持续流行,其抗原位点发生了一定程度的变异,未来应加强监测。Objective To understand the etiological examination results and hemagglutinin(HA)gene mutations of influenza B virus in Ningxia Region.Methods Forty-four influenza B virus isolates were sampled using the spatiotemporal sampling method from Ningxia Hui Autonomous Region from 2017 to 2023.RNA was extracted from virus isolates,and HA gene fragment was amplified using real-time reverse-transcription PCR(RT-PCR)assay,followed by sequencing of the amplified products.In addition,the sequencing results were subjected to bioinformatics analysis.Results Whole-genome sequencing of 44 influenza B virus isolates was performed.Mutations in antigenic sites were seen in 7 out of the 17BY lineage isolates relative to the B/California/12/2015 vaccine candidate,and there were R136G and L137H mutations in 120 antigenic epitopes,which all involved changes in receptor-binding sites.There were mutations in antigenic sites in 26 out of the 29 BV lineage isolates relative to theB/Colorado/06/2017vaccine candidate,which involved 11 receptor-binding sites in 4 antigenic epitopes.Mutations occurred in 3 receptor-binding sites,and no mutations were detected in potential glycosylation sites.All BY lineage virus isolates were clustered into the Clade 3,and all BV lineage virus isolates were clustered into the Clade 1A.Conclusions Influenza B continued to be prevalent in Ningxia Hui Autonomous Region from 2017 to 2023,and mutations occurred in antigenic sites.Intensified surveillance of influenza B is required.

关 键 词：乙型流感病毒 血凝素 基因变异 抗原位点 序列分析 

分 类 号：R373.1[医药卫生—病原生物学]