金纳米粒子/氧化石墨烯复合膜电化学免疫传感器的构建及其对鲜肉中马波沙星的检测  

Development of an electrochemical immunosensor based on gold nanoparticle/graphene oxide composite film and its detection of marbofloxacin in fresh meat

作　　者：陈秀金[1,2,3] 王雪晴 李兆周 王耀[1,2,3] 张敏 牛华伟[1,2,3] 刘恒言 安彪[1] CHEN Xiujin;WANG Xueqing;LI Zhaozhou;WANG Yao;ZHANG Min;NIU Huawei;LIU Hengyan;AN Biao(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471000,China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control,Luoyang 471000,China;National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang 471000,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]河南省食品绿色加工与质量安全控制国际联合实验室,河南洛阳471000 [3]食品加工与安全国家级实验教学示范中心,河南洛阳471000

出　　处：《食品与发酵工业》2024年第20期279-285,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31701694,31702218);洛阳市公益性行业科研专项(2202021A);河南省科技攻关项目(242102321131,232102320298);河南省青年人才托举工程项目(2020HYTP029);河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06)。

摘　　要：该文构建了一种金纳米粒子/氧化石墨烯复合膜修饰玻碳电极的电化学免疫传感器,用于鲜肉中马波沙星残留的检测。用电沉积法在电极表面合成金纳米粒子/氧化石墨烯复合膜,然后通过电化学法还原复合膜中的氧化石墨烯,固载抗体。采用循环伏安法对电极的修饰过程进行表征。用差分脉冲伏安法优化传感器的电化学检测条件。在最优条件下,对马波沙星进行定量检测。响应峰值电流变化量与马波沙星在0.5~400 ng/mL浓度范围内呈线性关系,检测限为0.05 ng/mL。传感器对猪肉、鸡肉和牛肉样品的检测限分别为0.09、0.10、0.09μg/kg,添加回收率为82.81%~101.99%,检测结果与超高效液相色谱-串联质谱法基本一致。结果表明,所建立的电化学免疫传感器可用于实际样品中马波沙星残留的检测。An electrochemical immunosensor based on glassy carbon electrode-modified gold nanoparticles/graphene oxide composite film was developed and applied to detect marbofloxacin residues in fresh meat.Gold nanoparticles/graphene oxide composite film was synthesized on the electrode surface by an electrodeposition method,and then the graphene oxide of composite film was reduced by an electrochemical method.The antibody was finally immobilized.The modified process of the electrode was characterized by cyclic voltammetry.The electrochemical detection conditions of immunosensor were optimized by differential pulse voltammetry.Under the optimum conditions,marbofloxacin was quantitatively determined.The change of peak current was linearly related to the concentration of marbofloxacin in the range of 0.5-400 ng/mL with a detection limit of 0.05 ng/mL.The detection limits in pork,chicken and beef samples were 0.09,0.10,and 0.09μg/kg respectively,and the recovery rates ranged from 82.81%to 101.99%.These results obtained are consistent with that of ultra-high performance liquid chromatography-tandem mass spectrometry.The results showed that the fabricated electrochemical immunosensor could be applied for the detection of marbofloxacin residues in real samples.

关 键 词：马波沙星 金纳米粒子 氧化石墨烯 电化学免疫传感器 鲜肉 

分 类 号：R28[医药卫生—中药学]