miR-154、miR-22与膀胱癌患者病理特征关系及预测TURBT后复发价值  

Relationship between miR-154 and miR-22 and the pathological characteristics of patients with bladder cancer and the value of predicting recurrence after TURBT

作　　者：姜崇浩 岳春光 李明[3] 巴晓 JIANG Chonghao;YUE Chunguang;LI Ming;BA Xiao(Department of Urology,Affiliated Hospital of North China University of Science and Technology,Tangshan,Hebei 063000,China;Department of Urology,the Second People′s Hospital of Fengrun District,Tangshan,Hebei 064099,China;Department of General Medicine,Central Hospital of Chengde,Chengde,Hebei 067000,China;Department of Emergency,Ningjin County Hospital,Xingtai,Hebei 055550,China)

机构地区：[1]华北理工大学附属医院泌尿外科,河北唐山063000 [2]河北省唐山市丰润区第二人民医院泌尿外科,河北唐山064099 [3]河北省承德市中心医院全科医学,河北承德067000 [4]河北省邢台市宁晋县医院急诊科,河北邢台055550

出　　处：《检验医学与临床》2024年第20期3068-3073,共6页Laboratory Medicine and Clinic

基　　金：河北省医学科学研究课题计划项目(20241331)。

摘　　要：目的探讨微小RNA-154(miR-154)、微小RNA-22(miR-22)与膀胱癌患者病理特征关系及预测经尿道膀胱肿瘤电切术(TURBT)后复发价值。方法选取2019年1月至2022年12月华北理工大学附属医院收治的150例膀胱癌患者作为研究对象,统计癌组织和癌旁组织、不同病理特征患者miR-154、miR-22表达水平,采用Spearman相关分析不同病理特征与miR-154、miR-22表达水平的相关性。根据患者TURBT后复发情况将其分为复发组和未复发组,比较两组miR-154、miR-22表达水平。绘制受试者工作特征(ROC)曲线分析miR-154、miR-22预测TURBT后复发的效能。采用卡普兰-迈耶生存曲线(K-M)进行不同miR-154、miR-22表达水平患者生存状况分析。结果膀胱癌患者癌组织miR-154、miR-22表达水平均低于癌旁组织,差异均有统计学意义(P<0.05)。不同临床分期、分化程度、淋巴结转移膀胱癌患者miR-154、miR-22表达水平比较,差异均有统计学意义(P<0.05)。Spearman相关分析结果显示,临床分期、淋巴结转移与miR-154、miR-22均呈负相关(P<0.05),分化程度与miR-154、miR-22呈正相关(P<0.05)。150例膀胱癌患者TURBT后共有15例失访,32例复发,103例未复发,分别纳入复发组和未复发组。复发组miR-154、miR-22表达水平均低于未复发组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,miR-154、miR-22预测TURBT后复发的曲线下面积(AUC)分别为0.823、0.817,当最佳截断值为0.44、0.71时,灵敏度为78.12%、87.50%,特异度为75.73%、63.11%。miR-154、miR-22联合预测TURBT后复发的AUC为0.854,灵敏度及特异度分别为90.62%、92.23%。150例膀胱癌患者TURBT后共有15例失访,124例生存,11例死亡。根据ROC曲线中miR-154、miR-22最佳截断值为界限,≤最佳截断值为低表达,>最佳截断值为高表达。K-M曲线分析结果显示,miR-154、miR-22低表达患者生存率分别为88.16%(67/76)、88.00%(66/75),低于miR-154、miR-22高表达患者的98.31%(58Objective To investigate the relationship between microrNA-154(miR-154)and microrNA-22(miR-22)and the pathological characteristics of patients with bladder cancer and the value of predicting recurrence after transurethral resection of bladder tumor(TURBT).Methods A total of 150 patients with bladder cancer admitted to the Affiliated Hospital of North China University of Science and Technology from January 2019 to December 2022 were selected as the research objects.The expression levels of miR-154 and miR-22 in cancer tissues and adjacent tissues and patients with different pathological characteristics were counted.Spearman correlation was used to analyze the correlation between different pathological features and the expression levels of miR-154 and miR-22.According to the recurrence after TURBT,the patients were divided into recurrence group and non-recurrence group,and the expression levels of miR-154 and miR-22 were compared between the two groups.The receiver operating characteristic(ROC)curve was drawn to analyze the efficacy of miR-154 and miR-22 in predicting recurrence after TURBT.Kaplan Meier survival curve(K-M)was used to analyze the survival status of patients with different miR-154 and miR-22 expression levels.Results The expression levels of miR-154 and miR-22 in bladder cancer tissues were lower than those in adjacent tissues,and the differences were statistically significant(P<0.05).There were significant differences in the expression levels of miR-154 and miR-22 in bladder cancer patients with different clinical stages,differentiation degrees and lymph node metastasis(P<0.05).Spearman correlation analysis showed that clinical stage and lymph node metastasis were negatively correlated with miR-154 and miR-22(P<0.05),and differentiation degree was positively correlated with miR-154 and miR-22(P<0.05).Among the 150 patients with bladder cancer after TURBT,15 cases were lost to follow-up,32 cases had recurrence,and 103 cases had no recurrence.They were divided into recurrence group and non-recurrence

关 键 词：膀胱癌 微小RNA-154 微小RNA-22 病理特征 经尿道膀胱肿瘤电切术 复发 

分 类 号：R737.14[医药卫生—肿瘤] R699.5[医药卫生—临床医学]