溃疡性结肠炎患者病情发展中ERK1/2信号通路变化及其与肠道机会性感染的关联  

Changes in ERK1/2 signaling pathway in the progression of ulcerative colitis and its association with intestinal opportunistic infections

作　　者：崔晓婷 芦杰 牛昊书[1] 赵翠娟[1] CUI Xiaoting;LU Jie;NIU Haoshu;ZHAO Cuijuan(Department of Gastroenterology,Baogang Hospital of Inner Mongolia,Baotou,Inner Mongolia 014010,China)

机构地区：[1]内蒙古包钢医院消化内科,内蒙古包头014010

出　　处：《中华全科医学》2024年第10期1636-1639,共4页Chinese Journal of General Practice

基　　金：内蒙古自然科学基金项目(2020MS08024)。

摘　　要：目的观察溃疡性结肠炎(UC)患者病情发展中细胞外调节蛋白激酶(ERK)1/2信号通路变化,并分析其与UC患者并发肠道机会性感染的关联。方法选取2021年5月—2023年5月内蒙古包头市包钢医院消化内科收治的105例UC患者作为研究组,同期体检健康者50例作为对照组,比较2组ERK-1、ERK-2 mRNA转录水平。根据是否发生肠道机会性感染将105例UC患者分为感染组、未感染组并筛选危险因素,分析其对UC患者并发肠道机会性感染的预测价值。结果研究组ERK-1、ERK-2 mRNA相对表达量均高于对照组(P<0.05),且其变化随UC患者病情进展而升高(P<0.05)。与未感染组比较,感染组疾病活动度重度率、血红蛋白、红细胞沉降率(ESR)水平及ERK-1 mRNA、ERK-2 mRNA相对表达量均较高(P<0.05),白蛋白水平较低(P<0.05)。重度、白蛋白降低及ERK-1 mRNA、ERK-2 mRNA表达上调是影响UC患者并发肠道机会性感染的独立危险因素,ERK-1 mRNA、ERK-2 mRNA及其联合应用模型预测UC患者并发肠道机会性感染的AUC分别为0.823、0.835、0.907。结论ERK-1、ERK-2表达水平随UC患者病情进展而升高,且其是影响UC患者发生肠道机会性感染的独立危险因素,两者联合预测UC患者并发肠道机会性感染的效能较高。Objective To observe the changes of extracellular regulatory protein kinase(ERK)1/2 signaling pathway in the progression of ulcerative colitis(UC)and analyze its association with intestinal opportunistic infection in UC patients.Methods A total of 105 UC patients admitted to the gastroenterology department of Baogang Hospital of Inner Mongolia from May 2021 to May 2023 were selected as the study group,and 50 healthy subjects were selected as the control group during the same period,the mRNA transcription levels of ERK-1 and ERK-2 were compared between the two groups.Ac-cording to the occurrence of intestinal opportunistic infection,105 UC patients were divided into an infected group and an uninfected group,and the risk factors were screened to analyze the predictive value of UC patients with intestinal oppor-tunistic infection.Results The mRNA relative expression levels of ERK-1 and ERK-2 in the study group were higher than those in the control group(P<0.05),and their changes increased with the progression of UC patients(P<0.05).Compared with the uninfected group,the disease activity severity rate,hemoglobin,erythrocyte sedimentation rate(ESR)levels,ERK-1 and ERK-2 mRNA transcription levels in the infected group were higher(P<0.05),and the al-bumin level was lower(P<0.05).Severity,decreased albumin and up-regulated expression of ERK-1 mRNA and ERK-2 mRNA were independent risk factors for intestinal opportunistic infections in UC patients.ROC curve results showed that the AUC of ERK-1 mRNA,ERK-2 mRNA,and their combined application model for predicting intestinal op-portunistic infection in UC patients were 0.823,0.835,and 0.907 respectively.Conclusion The expression levels of ERK-1 and ERK-2 increased with the progression of UC patients,and they were independent risk factors affecting the oc-currence of intestinal opportunistic infections in UC patients,the combined expression levels of ERK-1 and ERK-2 were highly effective in predicting intestinal opportunistic infections in UC patients.

关 键 词：溃疡性结肠炎 ERK1/2信号通路 肠道机会性感染 

分 类 号：R574.62[医药卫生—消化系统]