Establishing the link between D-mannose and juvenile grass carp(Ctenopharyngodon idella):Improved growth and intestinal structure associated with endoplasmic reticulum stress,mitophagy,and apical junctional complexes  

作　　者：Chong Zhang Lin Feng Pei Wu Yang Liu Xiaowan Jin Hongmei Ren Hua Li Fali Wu Xiaoqiu Zhou Weidan Jiang 

机构地区：[1]Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China [2]Fish Nutrition and Safety Production,University Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China [3]Key Laboratory of Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu 611130,China

出　　处：《Animal Nutrition》2024年第3期450-463,共14页动物营养（英文版）

基　　金：supported by the earmarked fund for CARS (CARS-45),and the National Key R&D Program of China (2019YFD0900200).

摘　　要：D-mannose,essential for protein glycosylation,has been reported to have immunomodulatory effects and to maintain intestinal flora homeostasis.In addition to evaluating growth performance,we examined the impact of D-mannose on the structure of epithelial cells and apical junction complexes in the animal intestine.All 1800 grass carp(16.20±0.01 g)were randomly divided into six treatments with six replicates of 50 fish each and fed with six different levels of D-mannose(0.52,1.75,3.02,4.28,5.50 and 6.78 g/kg diet)for 70 d.The study revealed that D-mannose increased feed intake(P 0.05).D-mannose supplementation at 1.75 g/kg increased crude protein content in fish and lipid production value(P<0.05).D-mannose supplementation at 4.28 g/kg increased intestinal length,intestinal weight and fold height of grass carp compared to the control group(P<0.05).This improvement may be attributed to the phosphomannose isomerase(PMI)-mediated enhancement of glycolysis.This study found that D-mannose supplementation at 4.28 or 3.02 g/kg reduced serum diamine oxidase activity or D-lactate content(P<0.05)and improved cellular and intercellular structures for the first time.The improvement of cellular redox homeostasis involves alleviating endoplasmic reticulum(ER)stress through the inositol-requiring enzyme 1(IRE1),RNA-dependent protein kinase-like ER kinase(PERK),and activating transcription factor 6(ATF6)signaling pathways.The alleviation of ER stress may be linked to the phosphomannomutase(PMM)-mediated enhancement of protein glycosylation.In addition,ubiquitin-dependent[PTEN-induced putative kinase 1(PINK1)/Parkin]and ubiquitin-independent[BCL2-interacting protein 3-like(BNIP3L),BCL2-interacting protein 3(BNIP3),and FUN14 domain containing 1(FUNDC1)]mitophagy may play a role in maintaining cellular redox homeostasis.The enhancement of intercellular structures includes enhancing tight junction and adherent junction structures,which may be closely associated with the small Rho GTPase protein(RhoA)/the Rho-associated protein kinase(R

关 键 词：Grass carp(Ctenopharyngodon idella) D-MANNOSE Intestine structure Endoplasmic reticulum stress MITOPHAGY Apical junctional complex 

分 类 号：S965.112[农业科学—水产养殖]