吡喃葡萄糖功能化氧化钼纳米酶的合成以及抗菌性能分析  

Synthesis and Antibacterial Performance Analysis of MoO_(3-x)Nanoenzyme Functionalized with Glucopyranose

作　　者：孙逸轩 刘泽众 白露 牛玉生 SUN Yi-Xuan;LIU Ze-Zhong;BAI Lu;NIU Yu-Sheng(College of Life Sciences,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学生命科学学院,青岛266071

出　　处：《分析化学》2024年第9期1316-1327,共12页Chinese Journal of Analytical Chemistry

基　　金：山东省自然科学基金项目(No.ZR2021MD094)资助。

摘　　要：过氧化物酶介导的化学动力学治疗(CDT)和光热疗法(PTT)协同抗菌策略已被证实可以有效抗菌,但是,由于活性氧(ROS)寿命短(<200 ns)和扩散距离不足(约20~200 nm)等因素,严重限制了对铜绿假单胞菌(Pseudomonas aeruginosa)的抗菌作用。本研究采用一锅水热法成功合成了具有类过氧化物酶活性和光热效应的吡喃葡萄糖功能化氧化钼(P-MoO_(3-x))纳米酶,并对其联合抗菌性能及生物安全性进行了分析验证。P-MoO_(3-x)纳米酶能够利用与吡喃葡萄糖和凝集素的特异性相互作用靶向铜绿假单胞菌表面,有效缩短了羟基自由基的作用距离,显著增强了对铜绿假单胞菌的抗菌效果。在光热作用下,P-MoO_(3-x)最佳反应温度为70℃,即使在低浓度(50μmol/L)H_(2)O_(2)存在下,也能释放更多的羟基自由基。体外抗菌实验结果表明,P-MoO_(3-x)抗菌系统对铜绿假单胞菌(106 CFU/mL)的失活效率超过99%。体内实验进一步证实,P-MoO_(3-x)在治疗耐甲氧西林金黄色葡萄球菌(MRSA)感染创面和促进伤口愈合方面疗效显著,并且不会对细胞产生毒性。本研究制备的P-MoO_(3-x)具有优良的抗菌效果和良好的生物相容性,在抗感染治疗中具有良好的应用前景。The synergistic antibacterial strategy of peroxidase mediated chemodynamic therapy(CDT)and photothermal therapy(PTT)has been proven to effectively resist bacteria.However,the antibacterial effect against Pseudomonas aeruginosa is severely limited due to the factors such as short lifespan of reactive oxygen species(ROS)(<200 ns)and limited diffusion distance(about 20‒200 nm).In this study,glucopyranose functionalized MoO_(3-x)(P-MoO_(3-x))nanoenzyme was successfully synthesized using a one-pot hydrothermal method.This nanoenzyme exhibited both peroxidase-like activity and a photothermal effect.The combined antibacterial performance and biological safety of P-MoO_(3-x) was analyzed and verified.By utilizing specific interactions with glucopyranose and lectin,P-MoO_(3-x) nanoenzyme could target the surface of Pseudomonas aeruginosa.This targeted approach effectively shortened the range of hydroxyl radicals,significantly enhancing the antibacterial effect against Pseudomonas aeruginosa.Under photothermal action,P-MoO_(3-x) could reach the optimal reaction effect at 70℃.Even at low concentrations of hydrogen peroxide(50μmol/L),it released more hydroxyl radicals.In vitro antibacterial analysis experiments demonstrated that the inactivation efficiency of the P-MoO_(3-x) antibacterial system against Pseudomonas aeruginosa(106 CFU/mL)exceeded 99%.Furthermore,in vivo experiments confirmed the significant therapeutic effects of P-MoO_(3-x) in treating methicillin-resistant Staphylococcus aureus(MRSA)infected wounds and promoting wound healing,without producing toxicity to cells.In conclusion,P-MoO_(3-x) exhibited excellent antibacterial ability and good biocompatibility,making it a promising anti-infective nanoenzyme with broad application prospects.

关 键 词：氧化钼 类过氧化物酶活性 光热效应 抑菌 

分 类 号：TB383.1[一般工业技术—材料科学与工程] Q814[生物学—生物工程]