益生菌对小鼠胆囊胆固醇结石形成过程中瘦素及肠道菌群的影响研究  

Effects of probiotics on leptin and intestinal flora druing the formation of gallstones in mice

作　　者：孙玥彤 薛荣泉 希龙夫 李勇利 Sun Yuetong;Xue Rongquan;Xi Longfu;Li Yongli(Inner Mongolia Clinical Medical College of Inner Mongolia Medical University,Hohhot 010017,China;Department of Hepatobiliary Pancreatic Splenic Surgery,Inner Mongolia Autonomous Region Peoples Hospital,Hohhot 010017,China;Inner Mongolia Academy of Medical Sciences,Hohhot 010017,China)

机构地区：[1]内蒙古医科大学内蒙古临床医学院,呼和浩特010017 [2]内蒙古自治区人民医院肝胆胰脾外科,呼和浩特010017 [3]内蒙古医学科学院,呼和浩特010017

出　　处：《国际外科学杂志》2024年第9期592-597,I0004,共7页International Journal of Surgery

基　　金：内蒙古自治区自然科学基金项目(2019MS08086);内蒙古自治区科技计划项目(2021GG0116);公立医院科研联合基金科技项目(2023GLLH0021)。

摘　　要：目的探讨益生菌对高脂饮食小鼠胆囊胆固醇结石形成的抑制作用及对瘦素和肠道菌群的影响。方法将40只8周龄SPF级雌性C57BL/6J小鼠适应性喂养1周后,采用随机数表法将小鼠分为基础盐水组(G-NS组,n=10)、基础益生菌组(G-Probiotics组,n=10)、高脂盐水组(L-NS组,n=10)、高脂益生菌组(L-Probiotics组,n=10)。G-NS组、G-Probiotics组喂饲基础饲料,L-NS组、L-Probiotics组喂饲致石饲料,同时给予G-Probiotics组、L-Probiotics组益生菌液(5×10^(9)CFU/mL,0.1 mL/10 g)灌胃,每日1次,持续8周,G-NS组、L-NS组灌胃等体积的0.9%氯化钠溶液。8周后实验结束,观察小鼠成石率,检测小鼠血清瘦素、总胆固醇、低密度脂蛋白胆固醇含量,收集粪便,分析小鼠肠道菌群组成多样性及丰度变化。计数资料采用百分率表示,组间比较采用Fisher确切概率法,正态分布的计量数据以均数±标准差(x±s)表示,组间比较采用单因素方差分析,肠道菌群部分则应用K-W秩和检验分析组间差异。结果G-NS组、G-Probiotics组未见胆囊结石,L-NS组、L-Probiotics组成石率分别为100%、60%,差异具有统计学意义(P<0.05)。G-NS组、L-NS组、G-Probiotics组、L-Probiotics组血清总胆固醇含量分别为(2.03±0.34)mmol/L、(4.75±0.76)mmol/L、(1.64±0.49)mmol/L、(3.66±0.62)mmol/L,血清低密度脂蛋白胆固醇含量分别为(0.57±0.10)mmol/L、(1.55±0.29)mmol/L、(0.73±0.37)mmol/L、(1.06±0.16)mmol/L,血清瘦素含量分别为(6.77±0.76)μg/L、(19.24±3.97)μg/L、(3.21±1.32)μg/L、(11.67±1.05)μg/L,血清总胆固醇、低密度脂蛋白胆固醇比较结果显示,L-NS组高于G-NS组和L-Probiotics组,L-Probiotics组高于G-Probiotics组,差异具有统计学意义(P<0.01);血清瘦素比较显示G-NS组、L-Probiotics组高于G-Probiotics组,L-NS组高于L-Probiotics组和G-NS组,差异具有统计学意义(P<0.01)。L-NS组与G-NS组相比肠道菌群多样性降低,有害菌属相对丰度增加,有益菌属相对丰度减�Objective To investigate the inhibitory effect of probiotics on cholesterol gallstone formation in mice fed a high-fat diet and its impact on leptin and intestinal flora.Methods Forty 8-week-old female C57BL/6J SPF mice were obtained.After one week of adaptive feeding,the mice were randomly assigned to G-NS group(n=10),G-Probiotics group(n=10),L-NS group(n=10),and L-Probiotics group(n=10).The G-NS group and G-Probiotics group were fed with standard diet,while the L-NS group and L-Probiotics group received lithogenic diet.Additionally,the G-Probiotics group and L-Probiotics group received a probiotic solution(5×10^(9) CFU/ml,0.1 mL/10 g)by gavage,once daily for 8 weeks.The G-NS group and L-NS group received an equal volume of normal saline by gavage.After 8 weeks,the stone formation rate of mice was observed,the serum leptin,total cholesterol(TC)and low-density lipoprotein cholesterol(LDL)contents of mice were detected,feces were collected and DNA was extracted to analyze the diversity and abundance of intestinal flora in mice.Count data were presented as percentages,Fisher′s exact probability test was used for comparisons,and measurement data with normal distribution were presented as mean±standard divation(x±s).One-way ANOVA was applied for inter-group comparisons,and the K-W rank sum test was utilized to analyze inter-group differences in the intestinal microbiota section.Results No gallstone was found in the G-NS group and G-Probiotics group.The stone formation rates of the L-NS group and the L-Probiotics group were 100%and 60%,respectively,with statistical significance(P<0.05).In the G-NS group,L-NS group,G-Probiotics group and L-Probiotics group the serum total cholesterol(TC)content were(2.03±0.34)mmol/L,(4.75±0.76)mmol/L,(1.64±0.49)mmol/L and(3.66±0.62)mmol/L,the serum low-density lipoprotein(LDL)cholesterol content was(0.57±0.10)mmol/L,(1.55±0.29)mmol/L,(0.73±0.37)mmol/L and(1.06±0.16)mmol/L,the serum leptin content was(6.77±0.76)μg/L,(19.24±3.97)μg/L,(3.21±1.32)μg/L and(11.67±1.05)μg

关 键 词：有益菌种 瘦素 胆囊结石病 小鼠 

分 类 号：R575.62[医药卫生—消化系统]