鸡内金药材及其伪品鉴别并质量研究  

Identification of Galli Gigerii Endothelium Corneum and Its Adulterants and Quality Study of the Medicinal Material

作　　者：邱韵静 童培珍 吴妙梨 邓立萍 杨丽 陈向东 李国卫 QIU Yunjing;TONG Peizhen;WU Miaoli;DENG Liping;YANG Li;CHEN Xiangdong;LI Guowei(Guangdong Yifang Pharmaceutical Co.,Ltd.·Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan,Guangdong,China 528244)

机构地区：[1]广东一方制药有限公司·广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《中国药业》2024年第20期57-62,共6页China Pharmaceuticals

基　　金：广东省基础与应用基础研究基金[2020B1515120033]。

摘　　要：目的建立鉴别鸡内金药材及其伪品的方法,并研究药材质量。方法采用超高效液相色谱(UPLC)法,色谱柱为Agilent Zorbax SB C_(18)柱(100 mm×2.1 mm,1.8μm),流动相为乙腈-水(4∶1,V/V)-0.1%磷酸水溶液(梯度洗脱),流速为0.3 mL/min,检测波长为260 nm,柱温为30℃,进样量为2μL。采用中药色谱指纹图谱相似度评价系统(2012版)绘制29批样品(正品18批,伪品11批)色谱图,确定共有峰并指认。采用SIMCA-P 14.0软件进行聚类分析,采用偏最小二乘法分析(PLS-DA)及主成分分析(PCA)法进行主成分分析与聚类。采用UPLC法测定指认出的成分含量,并比对鸡饲料主要成分与鸡内金药材的特征图谱,分析黄酮类成分来源。结果18批鸡内金药材特征图谱有9个共有峰,指认出其中4个黄酮类成分,包括染料木苷、大豆苷元、黄豆黄素、染料木素;聚类分析、PLS-DA法及PCA法均可将鸡内金药材及其伪品分别归类。鸡饲料主要成分与鸡内金药材图谱出现相同共有(特征)峰。结论所建立的方法可有效鉴别鸡内金药材及其伪品,初步推断鸡内金药材中黄酮类成分是因长期食用鸡饲料富集所得。Objective To establish a method for identifying the Galli Gigerii Endothelium Corneum and its adulterants,and to study the quality of the medicinal material.Methods The ultra-high-performance liquid chromatography(UPLC)method was adopted;the chromatographic column was the Agilent Zorbax SB C_(18)column(100 mm×2.1 mm,1.8μm),the mobile phase was acetonitrile-water(4∶1,V/V)-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 0.3 mL/min,the detection wavelength was 260 nm,the column temperature was 30℃,and the injection volume was 2μL.The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(version 2012)was used to plot the chromatogram of 29 batches of samples(18 certified products and 11 adulterants),the common peaks were marked and identified.The SIMCA-P 14.0 software was used for cluster analysis,the partial least squares-discriminant analysis(PLS-DA)and principal component analysis(PCA)were used for principal component analysis and clustering.The UPLC method was used to determine the contents of identified components,and the characteristic chromatogram of the main components of Gallus gallus domesticus feed was compared with that of Galli Gigerii Endothelium Corneum to analyze the sources of flavonoids.Results There were nine common peaks in the characteristic chromatogram of 18 batches of Galli Gigerii Endothelium Corneum,and four flavonoids were identified,including genistin,daidzein,glycitein and genistein.Galli Gigerii Endothelium Corneum and its adulterants can be distinguished by the cluster analysis,PLS-DA method and PCA method.There were common(characteristic)peaks in the chromatograms of main components of Gallus gallus domesticus feed and Galli Gigerii Endothelium Corneum.Conclusion The established method can effectively identify the Galli Gigerii Endothelium Corneum and its adulterants.It is preliminary inferred that the flavonoids in the Galli Gigerii Endothelium Corneum are induced by long-term enrichment of Gallus gallus domesticus

关 键 词：鸡内金 偏最小二乘法分析 聚类分析 主成分分析 含量测定 特征图谱 

分 类 号：R917[医药卫生—药物分析学]