陆地棉PHB基因家族鉴定与表达分析  

Identification and Expression Analysis of PHB Gene Family in Upland Cotton

作　　者：章妮 陈克龙[2] Zhang Ni;Chen Kelong(College of Life Science,Qinghai Normal University,Xining,810008;Qinghai Key Laboratory of Physical Geography and Environmental Processes,Qinghai Normal University,Xining,810008)

机构地区：[1]青海师范大学生命科学学院,西宁810008 [2]青海师范大学,青海省自然地理与环境过程重点实验室,西宁810008

出　　处：《分子植物育种》2024年第20期6633-6643,共11页Molecular Plant Breeding

基　　金：国家自然科学基金(41661023);第二次青藏高原综合科学考察研究(2019ZQKK0405);青海省创新平台建设专项(2020-ZJ-Y06)共同资助。

摘　　要：抑制素(prohibitin,PHB)是一类普遍存在的高度保守蛋白,与植物的生长发育和生物及非生物胁迫等生物过程密切相关。目前,PHB蛋白在拟南芥、水稻和大豆等多种植物中已被鉴定,然而在陆地棉中尚未进行系统鉴定。本研究通过生物信息学方法鉴定了28个PHB基因家族成员,保守基序和基因结构分析证实了PHB蛋白的系统发育关系。此外,启动子区域预测出与多种激素调节及胁迫相关的顺式作用元件,同时研究发现GhPHBs在植物不同部位及受到不同的非生物胁迫时的表达模式存在差异。本研究系统分析了陆地棉中GhPHBs基因的一般特性,为陆地棉及其他植物中PHB基因的功能特性研究提供了理论基础。Prohitin(PHB)is a widely distributed and highly conserved protein that is closely related to growth and development of plants and biological and abiotic stress processes.PHB protein has been identified in Arabidopsis thaliana,Oryza sativa and Glycine max,but has not been systematically identified in Gossypium hirsutum.In this study,28 members of the PHB gene family were identified by bioinformatics,the phylogenetic relationship of PHB protein was confirmed by conserved motif and gene structure analysis.In addition,the promoter region predicted a variety of hormone regulation and stress-related cis-elements,and GhPHBs were found to have different expression patterns in different parts of plants and under different abiotic stresses.This study systematically analyzed the general characteristics of GhPHBs in Gossypium hirsutum,and provided a theoretical basis for the study of the functional characteristics of PHB genes in Gossypium hirsutum and other plants.

关 键 词：陆地棉 PHB基因家族 系统进化 生物信息学 表达分析 

分 类 号：S562[农业科学—作物学]