基于高通量测序的两色金鸡菊转录组数据分析  

Transcriptome Data Analysis of Coreopsis tinctoria Based on High-throughput Sequencing

作　　者：孙浩男 刘冬云[1] 李明阳[1] 王鑫 田琳 吴芳芳 Sun Haonan;Liu Dongyun;Li Mingyang;Wang Xin;Tian Lin;Wu Fangfang(College of Landscape and Travel,Hebei Agricultural University,Baoding,071000;She Country Cities and Towns Administration Department of Garden,Handan,056400)

机构地区：[1]河北农业大学园林与旅游学院,保定071000 [2]河北省涉县城镇园林管理所,邯郸056400

出　　处：《分子植物育种》2024年第20期6683-6697,共15页Molecular Plant Breeding

基　　金：河北省重点研发计划现代种业科技专项(19226327D);涉县耐阴性地被植物的引种、应用及示范推广(194-220012021-2)共同资助。

摘　　要：本研究采用Illumina高通量测序技术对两色金鸡菊‘轮盘’(Coreopsis tinctoria'Roulette')叶片进行转录组测序,共获得有效数据7.53 Gb。经拼接、组装并去冗余后获得unigenes 36739条,平均长度为1197 bp。将unigene在七大功能数据库中对比,共有27372条(74.50%)成功获得注释,在NR和Swiss-Prot数据库中分别比对到20454和20849条;在GO数据库中,注释成功的18971条unigenes包含3大类别55个功能组;在KOG数据库注释成功的7189条unigenes可分为25个不同功能分类;通过KEGG数据库比对,7697条unigenes可分为5大类、19个亚类、129条代谢途径。基于同源序列对比得到22780条CDS,通过ESTScan预测后获得9148条CDS;利用MISA软件从6150条序列中搜索获得7627个SSR位点。本次转录组测序分析可为两色金鸡菊功能基因的表达、分子标记开发、新品种改良和培育提供一定理论参考。In this study,we obtanied about 7.53 Gb data in total from leaves transcriptome of Coreopsis tinctoria'Roulette'by Illumina high-throughput sequencing technology.After assembling and removing redundancy,the study obtained 36739 unigenes with an average length of 1197 bp.The study compared unigene sequences with seven functional databases,and a total of 27372(74.50%)were annotated successfully,in which 20454 in NR and 20849 in Swiss-Prot.18971 unigenes were annotated in GO database and divided into 55 function group of 3 categories.According to the function comparing with the KOG database,these unigenes from the obtained transcriptome were divided into 25 group.Moreover,7697 unigenes were broadly divided into 5 categories and 19 sub-categories,and could be further annotated to 129 KEGG pathway.22780 CDS were obtained based on homologous sequence comparison,and 9148 CDS were obtained by prediction with ESTScan.Based on homologous sequence comparison,22780 CDS were obtained,and 9148 CDS were obtained after the prediction by ESTScan.7627 SSR were detected from 6150 unigenes sequences by using MISA software.This transcriptome date analysis can provide a theoretical reference for the gene expression,development of SSR molecular markers,and the improvement and cultivation of new varieties in Coreopsis tinctoria.

关 键 词：两色金鸡菊 转录组 功能注释 高通量测序 

分 类 号：S681.9[农业科学—观赏园艺]