基于TCGA数据库分析结直肠癌中LOC676462表达及临床意义  

The expression and prognostic correlation of LOC676462 in colorectal cancer were analyzed based on TCGA database

作　　者：甄洪超[1] 谢俏[2] 曹邦伟[1] ZHEN Hongchao;XIE Qiao;CAO Bangwei(Department of Oncology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院肿瘤科,北京100050 [2]北京市垂杨柳医院消化内科,100020

出　　处：《临床肿瘤学杂志》2024年第6期539-544,共6页Chinese Clinical Oncology

摘　　要：目的探讨结直肠癌中lncRNA LOC676462表达及其与临床病理特征、预后的相关性。方法在癌症基因图谱(TCGA)数据库网站中下载结直肠癌RNA转录组数据及临床数据,进行LOC676462表达差异分析及生存分析。采用TIMER 2.0对LOC676462进行泛癌表达水平分析。收集首都医科大学附属北京友谊医院经手术治疗的100例结直肠癌肿瘤组织及癌旁组织标本,采用实时荧光定量PCR(qPCR)检测LOC676462表达量,分析LOC676462表达与结直肠癌者临床病理特征的关系。受试者特征工作曲线(ROC)分析LOC676462表达诊断结直肠癌的效能。Kaplan-meier法绘制总生存(OS)曲线。采用多因素Cox风险比例回归模型分析影响结直肠癌OS的因素。结果生物信息学分析结果显示,LOC676462在结直肠癌组织中高表达。100例结直肠癌组织中LOC676462表达量为3.421±2.250,高于癌旁组织的1±0.694,差异有统计学意义(P<0.05)。LOC676462表达与TNM分期、浸润深度及淋巴结转移有关(P<0.05)。ROC曲线分析表明,LOC676462表达诊断结直肠癌的曲线下面积为0.918(95%CI:0.882~0.955)。Kaplan-Meier结果显示,LOC676462高表达患者的5年总生存率为38%,低表达患者5年总生存率为65.9%,差异有统计学意义(P<0.05)。多因素Cox风险比例回归模型分析结果显示,TNM分期、浸润深度、肿瘤分化程度以及LOC676462表达是影响结直肠癌OS的独立因素(P<0.05)。结论长链非编码RNA LOC676462可作为促癌基因参与在结直肠癌的发生与发展,并且其可能是结直肠癌潜在的生物标志物,可作为预测结直肠癌患者生存预后的指标。Objective To investigate the expression of lncRNA LOC676462 in colorectal cancer and its correlation with clinicopathological features and prognosis.Methods The transcriptome and clinical data of colorectal cancer RNA were downloaded from the Cancer Gene Atlas(TCGA)database,and LOC676462 expression differential analysis and survival analysis were performed.The expression level of LOC676462 pancarcinoma was analyzed by TIMER 2.0.The expression of LOC676462 was detected by real-time quantitative fluorescent PCR(qPCR),and the relationship between the expression of LOC676462 and clinicopathological characteristics of colorectal cancer patients was analyzed.Receiver characteristic operating curve(ROC)was used to analyze LOC676462 expression in the diagnosis of colorectal cancer.The overall survival(OS)curve is plotted by Kaplan-meier method.Multivariate Cox proportional regression model was used to analyze the factors affecting OS in colorectal cancer.Results The bioinformatics analysis showed that LOC676462 was highly expressed in colorectal cancer tissues.The expression level of LOC676462 in 100 colorectal cancer tissues was 3.421±2.250,which was higher than 1±0.694 in adjacent tissues,and the difference was statistically significant(P<0.05).LOC676462 expression was correlated with TNM stage,invasion depth and lymph node metastasis(P<0.05).ROC curve analysis showed that the area under the curve of LOC676462 expression in the diagnosis of colorectal cancer was 0.918(95%CI:0.882-0.955).Kaplan-Meier results showed that the 5-year overall survival rate of patients with high expression of LOC676462 was 38%,while that of patients with low expression was 65.9%,the difference was statistically significant(P<0.05).Multivariate Cox proportional regression analysis showed that TNM stage,depth of invasion,degree of tumor differentiation and LOC676462 expression were independent factors affecting OS in colorectal cancer(P<0.05).Conclusion Long non-coding RNA LOC676462 can be used as an oncogene to participate in the occurrence

关 键 词：结直肠癌 长链非编码RNA TCGA数据库 预后 临床病理特征 

分 类 号：R735.3[医药卫生—肿瘤]