Good and bad dispositions between archaea and bacteria in the human gut:New insights from metagenomic survey and co-occurrence analysis  

作　　者：Francesco Candeliere Laura Sola Stefano Raimondi Maddalena Rossi Alberto Amaretti 

机构地区：[1]Department of Life Sciences,University of Modena and Reggio Emilia,41125 Modena,Italy [2]Biogest-Siteia,University of Modena and Reggio Emilia,41125 Modena,Italy

出　　处：《Synthetic and Systems Biotechnology》2024年第1期88-98,共11页合成和系统生物技术（英文）

基　　金：partially funded by PIANO NAZIONALE DI RIPRESA E RESILIENZA(PNRR)-MISSIONE 4 COMPONENTE 2,“Dalla ricerca all’impresa”INVESTIMENTO 1.3,Creazione di“Partenariati estesi alle universita,ai centri di ricerca,alle aziende per il finanziamento di progetti di ricerca di base”,finanziato dall’Unione europea-NextGenerationEU”-Progetto identificato con codice PE00000019,Titolo“HEAL ITALIA”-Spoke 5-CUP E93C22001860006 Avviso MUR DD.341 del 15.03.2022.

摘　　要：Archaea are an understudied component of the human microbiome.In this study,the gut archaeome and bacteriome of 60 healthy adults from different region were analyzed by whole-genome shotgun sequencing.Archaea were ubiquitously found in a wide range of abundances,reaching up to 7.2%.The dominant archaeal phylum was Methanobacteriota,specifically the family Methanobacteriaceae,encompassing more than 50%of Archaea in 50 samples.The previously underestimated Thermoplasmatota,mostly composed of Methanomassiliicoccaceae,dominated in 10 subjects(>50%)and was present in all others except one.Halobacteriota,the sole other archaeal phylum,occurred in negligible concentration,except for two samples(4.6-4.8%).This finding confirmed that the human gut archaeome is primarily composed of methanogenic organisms and among the known methanogenic pathway:i)hydrogenotrophic reduction of CO_(2) is the predominant,being the genus Methanobrevibacter and the species Methanobrevibacter smithii the most abundant in the majority of the samples;ii)the second pathway,that involved Methanomassiliicoccales,was the hydrogenotrophic reduction of methyl-compounds;iii)dismutation of acetate or methyl-compounds seemed to be absent.Co-occurrence analysis allowed to unravel correlations between Archaea and Bacteria that shapes the overall structure of the microbial community,allowing to depict a clearer picture of the human gut archaeome.

关 键 词：Gut microbiota ARCHAEA METAGENOME CO-OCCURRENCE Methanomassiliicoccus Methanobrevibacter smithii 

分 类 号：Q93[生物学—微生物学]