解淀粉芽孢杆菌F-1的分离鉴定及其胞外产物的功效活性研究  

Isolation and identification of Bacillus amyloliquefaciens F-1 and study on the efficacy and activity of its extracellular products

作　　者：张永涛 谢松霖 陈彬和 魏娜 吕智 屠一栋 ZHANG Yong-tao;XIE Song-lin;CHEN Bin-he;WEI Na;LV Zhi;TU Yi-dong(Shanghai Inoherb Cosmetics Co.,Ltd.,Shanghai 200444,China)

机构地区：[1]上海相宜本草化妆品股份有限公司,上海200444

出　　处：《日用化学品科学》2024年第10期33-38,共6页Detergent & Cosmetics

摘　　要：从腐乳中分离出一株解淀粉芽孢杆菌F-1,所分泌的胞外产物富含蛋白质和多糖,为了探究其胞外产物的潜在功效性和应用价值,通过抑菌实验、自由基清除实验和细胞实验探究其抗菌性、抗氧化性和抗炎等活性。实验结果表明,2%的胞外产物便能够抑制痤疮丙酸杆菌(Cutibacterium acnes)的生长和清除近50%的DPPH自由基(IC50=2.776%),在细胞水平上胞外产物表现出较强的抗氧化应激和抗炎的能力,0.01%对H_(2)O_(2)诱导的HFF-1活性氧的降低作用达到最强,胞外产物在0.1%内都能够显著地降低RAW264.7的白介素因子6和白介素因子10。Bacillus amyloliquefaciens strain F-1 was isolated from fermented bean curd in laboratory.The extracellular products secreted by B.amyloliquefaciens strain F-1 are rich in protein and polysaccharide.To explore the potential efficacy and application value of the extracellular products,the antibacterial activity,antioxidant activity and anti-inflammatory activity were investigated by antibacterial test,free radical scavenging test and cell test.The results showed that 2%of the extracellular products could inhibit the growth of Cutibacterium acnes and scavenge nearly 50%of DPPH free radicals(IC50=2.776%).At the cellular level,the extracellular products showed strong anti-oxidative stress and anti-inflammatory capabilities.The concentration of 0.01%had the strongest effect on the reduction of HFF-1 reactive oxygen species induced by H_(2)O_(2),and the extracellular products could significantly reduce the interleukin-factor 6 and interleukin-factor 10 of secreted by RAW264.7 within 0.1%concentration.

关 键 词：解淀粉芽孢杆菌 胞外产物 抑菌 抗氧化 抗炎 

分 类 号：TQ658[化学工程—精细化工]