超声辅助接骨木多糖提取工艺优化及其结构特性分析  

Optimization of Ultrasonically Assisted Elderberry Polysaccharide Extraction Process and Its Structural Characteristics Analysis

作　　者：魏鑫鑫 沈柯辰 姚俊修[2] 吴德军[2] 张仁堂[1] WEI Xin-xin;SHEN Ke-chen;YAO Jun-xiu;WU De-jun;ZHANG Ren-tang(College of Food Science and Engineering,Shandong Agricultural University/Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,Tai’an 271018,China;Shandong Academy of Forestry Science/Key Laboratory for Genetics and Breeding in Forest Trees of Shandong Province,Jinan 250014,China)

机构地区：[1]山东农业大学食品科学与工程学院/山东省高校食品加工技术与质量控制重点实验室,山东泰安271018 [2]山东省林业科学研究院/山东省林木遗传改良重点实验室,济南250014

出　　处：《中国食物与营养》2024年第9期45-51,共7页Food and Nutrition in China

基　　金：山东省重点研发计划(农业良种工程)“接骨木高产优质新品种选育”(项目编号:2020LZGC0905)。

摘　　要：目的:优化超声波辅助提取接骨木多糖的最佳提取工艺,并初步研究接骨木多糖的结构特性。方法:以接骨木果实(品种,青州15号)为原料,以料液比、超声温度、超声时间、超声功率为单因素,并在单因素基础上设计响应面优化超声波辅助提取工艺,采用紫外、红外光谱和高效液相色谱法对接骨木多糖进行初步结构鉴定。结果:接骨木多糖的最佳提取工艺为料液比1:20(g/mL)、超声温度75℃、超声功率500W、超声处理45min,接骨木果实多糖得率最高达3.17%。接骨木多糖(EFP)为α-糖苷键的吡喃型糖苷环骨架,其单糖组成主要为葡萄糖、阿拉伯糖、岩藻糖、半乳糖、半乳糖醛酸以及甘露糖,其比例为25.10:23.20:19.80:12.60:12.00:6.40,分子量为4.100×10^(5)Da。结论:本研究可为接骨木的开发、利用以及接骨木多糖的结构表征提供科学依据。【Objective】To optimize the optimal extraction process of ultrasonically assisted extraction of elderberry polysaccharides,and preliminarily study the structural characteristics of elderberry polysaccharides.【Method】Taking elderberry fruit(variety,Qingzhou No.15)as raw material,material-to-liquid ratio,ultrasonic temperature,ultrasonic time,and ultrasonic power as single factors,and designing the response surface on a single-factor basis to optimize the ultrasonic auxiliary extraction process,ultraviolet,infrared spectroscopy and high performance liquid chromatography were used for preliminary structural identification of butter polysaccharides.【Result】The optimal extraction process of elderberry polysaccharides was feed-to-liquid ratio 1:20(g/mL),ultrasonic temperature 75°C,ultrasonic power 500 W,ultrasonic treatment for 45 min,and the yield of elderberry fruit polysaccharide up to 3.17%.Elderberry polysaccharide(EFP)is a α-glycoside bonded pyran-glycoside ring skeleton,and its monosaccharide composition is mainly glucose,arabinose,fucose,galactose,galactolic acid and mannose,and its ratio is 25.10:23.20:19.80:12.60:12.00:6.40,and the molecular weight is 4.100×10^(5) Da.【Conclusion】This study provides a theoretical basis for the development and utilization of elderberry and the structural characterization of elderberry polysaccharides.

关 键 词：接骨木多糖 超声波辅助 响应面 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]