Two adjacent NLR genes conferring quantitative resistance to clubroot disease in Arabidopsis are regulated by a stably inherited epiallelic variation  

作　　者：Antoine Gravot Benjamin Lie gard Leandro Quadrana Florian Veillet Yoann Aigu Tristan Bargain Juliette Benejam Christine Lariagon Jocelyne Lemoine Vincent Colot Maria J.Manzanares-Dauleux Melanie Jubault 

机构地区：[1]IGEPP Institut Agro,INRAE,Universitede Rennes,35650 Le Rheu,France [2]Institut de Biologie de l’Ecole Normale Superieure(IBENS),Ecole Normale Superieure,Centre National de la Recherche Scientifique(CNRS),Institut National de la Santeet de la Recherche Medicale(INSERM),75005 Paris,France [3]IGEPP INRAE,Institut Agro,Universitede Rennes,29260 Ploudaniel,France [4]Present address:Aix-Marseille University,CEA,CNRS,Institute of Biosciences and Biotechnologies,BIAM Cadarache,13108 Saint-Paul-lez-Durance,France.

出　　处：《Plant Communications》2024年第5期148-162,共15页植物通讯（英文）

摘　　要：Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassica-ceae.Using a combination of quantitative trait locus(QTL)fine mapping,CRISPR-Cas9 validation,and extensive analyses of DNA sequence and methylation patterns,we revealed that the two adjacent neigh-boring NLR(nucleotide-binding and leucine-rich repeat)genes AT5G47260 and AT5G47280 cooperate in controlling broad-spectrum quantitative partial resistance to the root pathogen P.brassicae in Arabidopsis and that they are epigenetically regulated.The variation in DNA methylation is not associated with any nucleotide variation or any transposable element presence/absence variants and is stably inherited.Vari-ations in DNA methylation at the Pb-At5.2 QTL are widespread across Arabidopsis accessions and corre-late negatively with variations in expression of the two genes.Our study demonstrates that natural,stable,and transgenerationally inherited epigenetic variations can play an important role in shaping resistance to plant pathogens by modulating the expression of immune receptors.

关 键 词：METHYLATION CLUBROOT Plasmodiophora brassicae AT5G47260 AT5G47280 ADR1-L3 ddm1 

分 类 号：Q94[生物学—植物学]