Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation  

作　　者：Junfeng Cao Zhiwen Chen Luyao Wang Ning Yan Jialing Lin Lipan Hou Yongyan Zhao Chaochen Huang Tingting Wen Chenyi Li Saeed ur Rahman Zehui Liu Jun Qiao Jianguo Zhao Jie Wang Yannan Shi Wei Qin Tong Si Yuliang Wang Kexuan Tang 

机构地区：[1]Frontiers Science Center for Transformative Molecules,Joint International Research Laboratory of Metabolic and Developmental Sciences,Plant Biotechnology Research Center,Fudan-SJTU Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Engineering Research Center of Coal-based Ecological Carbon Sequestration Technology of the Ministry of Education,Key Laboratory of Graphene Forestry Application of National Forest and Grass Administration,Shanxi Datong University,Datong 037009,China [3]National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [4]Hainan Institute,Zhejiang University,Yongyou Industry Park,Yazhou Bay Sci-Tech City,Sanya 572000,China [5]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [6]Shandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China [7]Institute of Millet Crops,Hebei Academy of Agriculture&Forestry Sciences/Hebei Branch of China National Sorghum Improvement Center,Shijiazhuang 050035,China

出　　处：《Plant Communications》2024年第3期31-46,共16页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China (82274047 and 52071192);the National Key R&D Program of China (2018YFA0900600);the Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education (MJST2023-3);the China Postdoctoral Science Foundation (2023M732232);SJTU Trans-med Awards Research (20190104);supported in part by the Bill&Melinda Gates Foundation (OPP1199872 and INV-027291).

摘　　要：We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological compound.Seedling growth and biomass were promoted when A.annua was cultivated with low concentrations of graphene,an effect which was attributed to a 1.4-fold increase in nitrogen uptake,a 15%–22%increase in chlorophyllfluorescence,and greater abun-dance of carbon cycling–related bacteria.Exposure to 10 or 20 mg/L graphene resulted in a�60%increase in H2O2,and graphene could act as a catalyst accelerator,leading to a 9-fold increase in catalase(CAT)ac-tivity in vitro and thereby maintaining reactive oxygen species(ROS)homeostasis.Importantly,graphene exposure led to an 80%increase in the density of glandular secreting trichomes(GSTs),in which artemisinin is biosynthesized and stored.This contributed to a 5%increase in artemisinin content inmature leaves.Inter-estingly,expression of miR828 was reduced by both graphene and H2O2 treatments,resulting in induction of its target gene AaMYB17,a positive regulator of GST initiation.Subsequent molecular and genetic assays showed that graphene-induced H2O2 inhibits micro-RNA(miRNA)biogenesis through Dicers and regulates the miR828–AaMYB17 module,thus affecting GST density.Our results suggest that graphene may contribute to yield improvement in A.annua via dynamic physiological processes together with miRNA regulation,and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.

关 键 词：Artemisia annua ARTEMISININ glandular secreting trichomes GSTs MIRNA GRAPHENE 

分 类 号：Q94[生物学—植物学]