The GmSTF1/2–GmBBX4 negative feedback loop acts downstream of blue-light photoreceptors to regulate isoflavonoid biosynthesis in soybean  

作　　者：Zhaoqing Song Fengyue Zhao Li Chu Huan Lin Yuntao Xiao Zheng Fang Xuncheng Wang Jie Dong Xiangguang Lyu Deyue Yu Bin Liu Junyi Gai Dongqing Xu 

机构地区：[1]National Center for Soybean Improvement,State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China [2]Beijing Key Laboratory of Environmentally Friendly Management of Fruit Diseases and Pests in North China,Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China [3]Institute of Crop Science,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [4]State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Plant Communications》2024年第2期198-214,共17页植物通讯（英文）

基　　金：supported by the Natural Science Foundation of Jiangsu for Distinguished Young Scholars (BK20211525);the National Natural Science Foundation of China (32270256,31970258);the Core Technology Development for Breeding Program of Jiangsu Province (JBGS-2021-014);the Jiangsu Collaborative Innovation Center for Modern Crop Production (to D.X.);Nanjing Agricultural University (start-up funding to D.X.).

摘　　要：Isoflavonoids,secondary metabolites derived from the phenylalanine pathway,are predominantly bio-synthesized in legumes,especially soybean(Glycine max).They are not only essential for plant responses to biotic and abiotic stresses but also beneficial to human health.In this study,we report that light signaling controls isoflavonoid biosynthesis in soybean.Blue-light photoreceptors(GmCRY1s,GmCRY2s,GmPHOT1s,and GmPHOT2s)and the transcription factors GmSTF1 and GmSTF2 promote isoflavonoid accumulation,whereas the E3 ubiquitin ligase GmCOP1b negatively regulates isoflavonoid biosynthesis.GmPHOT1s and GmPHOT2s stabilize GmSTF1/2,whereas GmCOP1b promotes the degradation of these two proteins in soybean.GmSTF1/2 regulate the expression of approximately 27.9%of the genes involved in soybean isoflavonoid biosynthesis,including GmPAL2.1,GmPAL2.3,and GmUGT2.They also repress the expression of GmBBX4,a negative regulator of isoflavonoid biosynthesis in soybean.In addition,GmBBX4 physically interacts with GmSTF1 and GmSTF2 to inhibit their transcriptional activation activity toward target genes related to isoflavonoid biosynthesis.Thus,GmSTF1/2 and GmBBX4 form a negative feedback loop that acts downstream of photoreceptors in the regulation of isoflavonoid biosynthesis.Our study provides novel insights into the control of isoflavonoid biosynthesis by light signaling in soybean and will contribute to the breeding of soybean cultivars with high isoflavonoid content through genetic and metabolic engineering.

关 键 词：PHOTORECEPTOR light signaling ISOFLAVONOID GmSTF GmBBX4 SOYBEAN 

分 类 号：Q945[生物学—植物学]