Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam Ruditapes philippinarum in dose-and time-dependent manner  

作　　者：Ming CONG Zhaoshun LI Wenwen TIAN Yuanmei LI Jiasen LÜ 

机构地区：[1]School of Ocean,Yantai University,Yantai 264005,China [2]School of Life Sciences,Yantai University,Yantai 264005,China

出　　处：《Journal of Oceanology and Limnology》2024年第5期1634-1650,共17页海洋湖沼学报（英文）

基　　金：Supported by the Natural Science Foundation of Shandong Province(No.ZR 2023 MD 059);the National Natural Science Foundation of China(No.41876135)。

摘　　要：Previous studies have revealed that ammonia nitrogen has several adverse effects on clam Ruditapes philippinarum.However,knowledge is lacking regarding the related proteins involved in the toxicological responses,which is vital to elucidate the underlying mechanism of ammonia nitrogen.In this study,clams R.philippinarum were exposed to ammonia nitrogen for 21 d at two environmentally relevant concentrations.The tandem mass tags approach(TMT)was applied to assay the differentially expressed proteins(DEPs)in clam gill tissues on the 3 rd and 21 st day.Finally,a total of 7263 proteins were identified.Bioinformatics analyses revealed that clam protein profiles changed in dose-and time dependent manner after ammonia nitrogen exposure.We inferred that the clams may face heavy challenges after ammonia exposure,such as unbalanced gender ratio,lysosomal disease,energy lack,neurological disorders,altered glutamine metabolism,increased lipid synthesis,and impaired immunity.Variation profiles of enzyme activities of glutaminase and glutamine synthase provided direct evidence to verify the related inference from proteome data.Most of the inferred toxic effects merit further study.This study identified important proteins related to ammonia nitrogen toxicity in the clam and indicated the severe stress of marine ammonia pollution on the healthy development of mollusc aquaculture.

关 键 词：ammonia nitrogen Ruditapes philippinarum proteomic analysis TOXICITY 

分 类 号：S917.4[农业科学—水产科学] X55[环境科学与工程—环境工程] X503.225