Foliar spraying of chlorpyrifos induces morphometric changes in Glycine max(L.)and shifts native soil microbiome  

作　　者：Aman Raj Muneer Ahmad Malla Ashwani Kumar Pramod Kumar Khare Sheena Kumari 

机构地区：[1]Metagenomics and Secretomics Research Laboratory,Department of Botany,Dr.Harisingh Gour University(A Central University),Sagar,470003,M.P.,India [2]Institute for Water and Wastewater Technology,Durban University of Technology,Durban,4001,South Africa [3]Metagenomics and Secretomics Research Laboratory,Department of Botany,University of Allahabad(A Central University),Prayagraj,211002,U.P.,India [4]Department of Botany,Ecology laboratory,Dr.Harisingh Gour Vishwavidyalaya(A Central University),Sagar,Madhya Pradesh,470003,India

出　　处：《Emerging Contaminants》2024年第3期86-102,共17页新兴污染物（英文）

基　　金：support obtained through the project grant of(CRG/2021/003696);New Delhi,Govt of India.SK&MM would like to acknowledge the National Research Foundation of South Africa(UID number 129358)for financial support.

摘　　要：Pesticide usage alters plant growth,and development and disturbs native soil microbiome.The present study was carried out to evaluate the effect of Chlorpyrifos(CP-20%EC)on the growth and development of soybean(Glycine max),soil fertility,and soil microbiome.We conducted a pot experiment to assess the impact of different concentrations of chlorpyrifos;recommended dose(RD)and higher dose rates(2×and 4×RD).Chlorpyrifos application significantly altered the growth parameters in soybean(p<0.05).At higher concentrations(2×and 4×RD)shoot length(17.16±1.04;14.33±1.15,p<0.05),root length(3.40±0.36;2.66±0.28,p<0.050),leaf count(6.33±0.57;2.66±0.57;p<0.05)reduced significantly compared to RD and Control groups.Similarly at these concentrations(2×and 4×RD),significant reduction in fresh shoot weight(0.86±0.008;0.66±0.002,p<0.05),dry shoot weight(0.14±0.001;0.13±0.003,p<0.05)and root fresh weight(0.13±0.001;0.09±0.003,p<0.05)and root dry weight(0.030±0.0005,0.037±0.003,p<0.05)was observed compared to the RD and control group.Notably,a significant increase in root and shoot morphometric parameters was observed in RD compared to control(p<0.05).All the physio-chemical properties such as pH(8.51±0.82),moisture content(33.73±0.04),and electrical conductivity(3.44±0.03)were higher in CP-treated soils compared to control.Similarly,nutrient content such as nitrogen,carbon,and hydrogen were significantly reduced in treated soils compared to control.Elemental analysis showed higher concentrations of As,Cd,and Pb in CP-treated soil(8.7 mg/g,0.254 mg/g,and 16.477 mg/g,respectively)compared to untreated soil(8.09 mg/g,0.228 mg/g,and 15.25 mg/g,respectively).We also assessed soil metabolic activity and diversity using Community-level Physiological Profiling(CLPP).CP-treated soil exhibited significantly lesser microbial diversity,with lesser metabolic activity.Metagenomic analysis revealed a shift in bacterial community composition,with Pseudomonadota and Bacteroidota dominating CP-treated soil,while Actinomycetota,

关 键 词：CHLORPYRIFOS Glycine max Proline Metabolic activity CLPP METAGENOMICS Bacterial microbiome 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]