小鼠蓝斑内酪氨酸羟化酶阳性神经元向丘脑室旁核投射的形态学研究  

Morphological study on projections of tyrosine hydroxylase positive neurons in locus coeruleus to paraventricular nucleus of the thalamus in mice

作　　者：张蓬鑫 朱慧 彭霏 吕培源 冯慧洁 薛美琪 薛懿珈 董玉琳 ZHANG Pengxin;ZHU Hui;PENG Fei;LYU Peiyuan;FENG Huijie;XUE Meiqi;XUE Yijia;DONG Yulin(Department of Anatomy,Histology and Embryology,K.K.Leung Brain Research Centre,Air Force Medical University,Xi’an,710032;The Fourth Student Brigade of Basic Medical College,Air Force Medical University,Xi’an,710032;Department of Anatomy,Medical College,Yan’an University,Yan’an,716000;Institute of Medical Research,Northwestern Polytechnical University,Xi’an,710072;The Fifth Student Brigade of Basic Medical College,Air Force Medical University,Xi’an,710032 China)

机构地区：[1]空军军医大学基础医学院人体解剖学教研室暨梁銶琚脑研究中心,西安710032 [2]空军军医大学基础医学院学员四大队,西安710032 [3]延安大学基础医学院人体解剖学教研室,延安716000 [4]西北工业大学医学研究院,西安710072 [5]空军军医大学基础医学院学员五大队,西安710032

出　　处：《神经解剖学杂志》2024年第4期405-412,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32271045);陕西省重点研发计划一般项目(2023-YBSF-156)。

摘　　要：目的:观察蓝斑(LC)内酪氨酸羟化酶(TH)阳性神经元向丘脑室旁核(PVT)内速激肽-1(TAC1)阳性神经元的投射,并从形态学上观察该通路是否参与伤害性信息的传递或调控。方法:利用TAC1-ires-Cre与Rosa26:CAG-LSL-tdTomato(Ai9)小鼠杂交,繁育TAC1-ires-Cre::Ai9小鼠,并制备坐骨神经分支损伤(SNI)模型,观察PVT内TAC1阳性神经元与FOS免疫阳性产物的共存情况,以及TAC1/FOS双标神经元与TH阳性轴突终末之间的密切接触。其次在C57BL/6小鼠PVT内注入荧光金(FG),在显微镜下观察LC内TH阳性神经元与FG逆标神经元的共标情况。最后在TAC1-ires-Cre小鼠的PVT注射RV逆行跨单突触三联病毒,观察LC内TH阳性神经元与RV逆标神经元之间的共存情况。结果:在TAC1-ires-Cre::Ai9小鼠脑内,TAC1阳性神经元为红色荧光所标记。SNI模型下PVT内可观察到部分TAC1阳性神经元同时表达FOS蛋白,且与TH阳性轴突终末之间形成密切接触。将FG注射入小鼠PVT后,在LC内可观察到大量的FG逆标神经元,且部分FG标记神经元同时表达TH阳性。利用RV逆行跨单突触示踪病毒,也可在LC内观察到PVT内TAC1阳性神经元的突触前神经元,且大部分突触前神经元为TH阳性。结论:LC内TH阳性神经元可向PVT内TAC1阳性神经元发出投射,形成LC^(TH+)-PVT^(TAC1+)神经通路,该通路可以被伤害性信息激活,提示其可能在痛觉信息的传递或调控过程中发挥一定的作用。Objective:To observe the projections from tyrosine hydroxylase(TH)positive neurons in locus coeruleus(LC)to tachykinin-1(TAC1)neurons in paraventricular nucleus of the thalamus(PVT),and morphologically determine whether they are involved in transmission and modulation of nociceptive information.Methods:TAC1-ires-Cre mice were hybridized with Rosa26:CAG-LSL-tdTomato(Ai9)mice.And spared nerve injury(SNI)induced neuropathic pain model was established with TAC1-ires-Cre::Ai9 mice to observe the colocalization of TAC1 and Fos and the close appositions between TAC1/FOS double-labeled neurons and TH positive axonal terminals.The distribution of the TH positive neurons and FG retrogradely labeled neurons were observed in the LC after Fluorogold(FG)was injected into the PVT.Finally,the coexistences of TH positive neurons and RV labeled neurons in the LC were observed after injection of RV-mediated retrograde tracing system.Results:TAC1 positive neurons were shown with red fluorescence in TAC1-ires-Cre::Ai9 mice.TAC1/FOS double-labeled neurons were found in the PVT of the SNI model.Some TAC1/FOS double labeled neurons made close appositions with TH positive axonal terminals.FG retrogradely labeled neurons were observed in the LC after FG injected into the PVT,and some of the FG labeled neurons coexisted with TH positive neurons.Using RV retrograde transsynaptic tracing virus,the results showed that presynaptic neurons of TAC1 positive neurons in the PVT were found in the LC,and most of the presynaptic neurons were TH positive neurons.Conclusion:TH positive neurons in the LC project to TAC1 positive neurons of the PVT,forming LC ^(TH+)-PVT ^(TAC1+)neural circuit,which were activated by nociceptive information.It demonstrates that this pathway plays a role in pain transmission or regulation.

关 键 词：蓝斑 丘脑室旁核 酪氨酸羟化酶 速激肽-1 伤害性信息 小鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]