光栓塞法建立脑卒中后吞咽障碍小鼠模型  

Establishment of a post-stroke dysphagia mouse model by photothrombosis method

作　　者：田聪 饶泽华 饶彤 陆梦 陈安坤 刘欣 慕容志苗 岳增辉[1] TIAN Cong;RAO Zehua;RAO Tong;LU Meng;CHEN Ankun;LIU Xin;MURONG Zhimiao;YUE Zenghui(School of Acupuncture,Massage and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410208

出　　处：《神经解剖学杂志》2024年第4期452-458,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82205298,81673886);湖南省自然科学基金(2023JJ30462);湖南省中医药管理局中医药科研一般项目(B2024007);湖南中医药大学校级科研项目(Z2023XJYQ07,Z2023YYJJ02)。

摘　　要：目的:建立一种可行的小鼠脑卒中后吞咽障碍(PSD)模型。方法:30只C57BL/6雄性小鼠随机分成假手术(Sham)组和模型(PSD)组,光栓塞(PT)法制作PSD小鼠模型,假手术组仅尾静脉注射玫瑰红。分别于第1、3、7 d采用激光散斑成像技术测定小鼠的脑血流量,采用TTC染色法检测脑梗死面积比率,采用多导生理记录仪MP160记录小鼠吞咽的在体吞咽肌群肌电面积,采用4 min饮水实验测量小鼠的饮水功能,记录小鼠的体重变化。结果:小鼠各时间点脑血流量均下降,第1 d脑血流量急剧下降,第3、7 d脑血流量逐渐恢复,侧支循环建立,脑梗死面积逐渐减小;与Sham组比较,PSD组小鼠第1、3 d肌电面积均减小(P<0.05),但呈逐渐恢复趋势,第7 d与Sham组比较无显著性差异,第1、3、7 d的4 min内饮水量、体重均有所下降(P<0.05)。结论:小鼠出现一定程度的吞咽障碍症状,有望成为PSD的转化模型。Objective:To establish a feasible mouse model of post-stroke dysphagia(PSD).Methods:Thirty C57BL/6 male mice were randomly divided into a sham-operated group(Sham)and a model group(PSD),and the PSD mouse model was made by the photothrombosis method(PT)method,and the sham-operated group was only injected with rose bengal staining solution in the tail vein.The cerebral blood flow of the mice was measured by laser scatter imaging,the ratio of cerebral infarct area was detected by TTC staining,the electromyographic area of the in vivo pharyngeal muscle group of mice swallowing was recorded by a multi-conductor physiological recorder MP160,the drinking function of the mice was measured by the 4-min water drinking experiment,and the weight changes were recorded,respectively,at 1,3,and 7 d.Results:Cerebral blood flow decreased at all time points,with a sharp drop in cerebral blood flow at 1 d,gradual recovery of cerebral blood flowat 3 and 7 d,establishment of collateral circulation,and gradual reduction of cerebral infarction area;compared with the Sham group,the myoelectric area of the PSD group was reduced at 1 and 3 d(P<0.05),but with a trend of gradual recovery,and there was no significant difference between the PSD group and the Sham group at 7 d,and water consumption and weight decreased at 4 min at 1,3,and 7 d(P<0.05).Conclusion:The mice showed some degree of dysphagia symptoms and are expected to be a translational model for PSD.

关 键 词：光栓塞 脑卒中后吞咽障碍 吞咽障碍 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]