白藜芦醇联合施万细胞样细胞对大鼠坐骨神经损伤的修复作用  

Repair effect of resveratrol combined with Schwann cell-like cells on sciatic nerve injury in rats

作　　者：刘杏 郑玲 刘雨 易黎明 饶利兵 鞠晓军 LIU Xing;ZHENG Ling;LIU Yu;YI Liming;RAO Libing;JU Xiaojun(Department of Human Anatomy,Hunan University of Medicine,Huaihua 418000,China;Clinical Skills Training Center,Hunan University of Medicine,Huaihua 418000,China)

机构地区：[1]湖南医药学院基础医学院人体解剖学教研室,怀化418000 [2]湖南医药学院医学信息与工程学院临床技能实训中心,怀化418000

出　　处：《神经解剖学杂志》2024年第4期485-491,共7页Chinese Journal of Neuroanatomy

基　　金：教育厅科学研究项目(22B1033);中医药科研计划(2021095);大学生创新创业训练计划(2020-4272)。

摘　　要：目的:探讨白藜芦醇联合脂肪源性干细胞(ADSCs)分化的施万细胞样细胞(SCLCs)对大鼠坐骨神经损伤的修复作用。方法:ADSCs原代培养并向SCLCs诱导分化,扫描电镜观察细胞形态;Western Blot方法检测S100钙结合蛋白β(S100β)、p75神经营养素受体(p75NTR)、胶质纤维酸性蛋白(GFAP)表达。大鼠随机分为对照组(Control)、施万细胞样细胞组(SCLCs)、白藜芦醇组(Res)、白藜芦醇+施万细胞样细胞组(Res+SCLCs),坐骨神经损伤模型造模成功后8周通过足迹实验检测各组坐骨神经功能指数(SFI);von Frey丝刺激针测定机械缩足阈值(MWT);称重法测定胫前肌湿重比率(WR);Western Blot、RT-qPCR方法检测损伤处神经营养素-3(NT-3)、神经生长因子(NGF)、类胰岛素生长因子-1(IGF-1)和脑源性神经营养因子(BDNF)表达情况。结果:ADSCs诱导8 d后细胞两极细长,细胞外成分增多;S100β、p75NTR、GFAP蛋白高表达。给予SCLCs、Res及Res+SCLCs治疗后,治疗组SFI、WR明显优于Control组(P<0.05);Res+SCLCs组、SCLCs组大鼠MWT降低(P<0.05)。Western Blot结果显示:Res+SCLCs组大鼠NT-3、IGF-1、NGF、BDNF蛋白表达高于其余各组(P<0.05);SCLCs组大鼠NT-3、NGF、BDNF蛋白表达高于Control组(P<0.05);Res组大鼠NT-3、NGF蛋白表达高于Control组(P<0.05)。RT-qPCR结果显示:Res+SCLCs组大鼠NT-3、IGF-1、NGF、BDNF mRNA高表达;SCLCs组大鼠NT-3、IGF-1、NGF、BDNF mRNA表达高于Control组(P<0.05);Res组大鼠IGF-1、NGF mRNA表达高于Control组(P<0.05)。结论:Res联合ADSCs分化的SCLCs对大鼠坐骨神经损伤具有良好的修复作用。Objective:To explore the repair effect of resveratrol combined with Schwann cell-like cells(SCLCs)differentiated from adipose-derived stem cells(ADSCs)on sciatic nerve injury in rats.Methods:ADSCs were primarily cultured and induced to differentiate into SCLCs.Cell morphology was observed by scanning electron microscopy.Western Blot method was used to detect the expressions of S100 calcium-binding proteinβ(S100β),p75 neurotrophin receptor(p75NTR),and glial fibrillary acidic protein(GFAP).Rats were randomly divided into Control group(Control),Schwann cell-like cell group(SCLCs),resveratrol group(Res),and resveratrol+Schwann cell-like cell group(Res+SCLCs).Eight weeks after the successful establishment of the sciatic nerve injury model,the sciatic nerve function index(SFI)of each group was detected by footprint experiment;the mechanical withdrawal threshold(MWT)was measured by von Frey filament stimulation needle;The wet weight ratio(WR)of the tibialis anterior muscle was determined by weighing method;Western Blot and RT-qPCR methods were used to detect the expressions of neurotrophin-3(NT-3),nerve growth factor(NGF),insulin-like growth factor-1(IGF-1),and brain-derived neurotrophic factor(BDNF)at the injury site.Results:After 8 days of induction of ADSCs,the cells had elongated poles and increased extracellular components;S100β,p75NTR,and GFAP proteins were highly expressed.After treatment with SCLCs,Res,and Res+SCLCs,the SFI and WR of the treatment groups were significantly better than those of the Control group(P<0.05);the MWT of rats in the Res+SCLCs group and SCLCs group was reduced(P<0.05).Western Blot results showed that the expressions of NT-3,IGF-1,NGF,and BDNF proteins in rats in the Res+SCLCs group were higher than those in other groups(P<0.05);The expressions of NT-3,NGF,and BDNF proteins in rats in the SCLCs group were higher than those in the Control group(P<0.05);the expressions of NT-3 and NGF proteins in rats in the Res group were higher than those in the Control group(P<0.05).RT-qPCR results sh

关 键 词：坐骨神经 施万细胞样细胞 脂肪源性干细胞 白藜芦醇 分化 大鼠 

分 类 号：R745[医药卫生—神经病学与精神病学]