唾液宿主关联乳杆菌ATCC 11741益生特性  

Probiotic characteristics of Ligilactobacillus salivarius ATCC 11741

作　　者：梅雪锋 华德平 田雨菁 黄金海[1] 张蕾[1] MEI Xuefeng;HUA Deping;TIAN Yujing;HUANG Jinhai;ZHANG Lei(School of Life Sciences,Faculty of Medicine,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学医学部生命科学学院,天津300072

出　　处：《微生物学报》2024年第10期3901-3915,共15页Acta Microbiologica Sinica

基　　金：国家合成生物技术创新中心(TSBICIP-KJGG-014)。

摘　　要：【目的】部分唾液宿主关联乳杆菌(Ligilactobacillus salivarius)菌株具备优良的益生特性和较好的应用潜力。以唾液宿主关联乳杆菌ATCC11741菌株为研究对象,探究其生长特性、耐受性和黏附能力,以期为唾液宿主关联乳杆菌的有效利用提供理论基础。【方法】利用形态观察和16SrRNA基因测序确认唾液宿主关联乳杆菌ATCC11741的准确性;通过监测生长曲线和产酸曲线来探究其生长特性;此外,还进行了一系列耐受性试验,包括耐酸、耐碱、耐胆盐、耐高渗和耐温测试,以评估其耐受能力;最后,借助自聚集和疏水性试验来间接测定其黏附性。【结果】唾液宿主关联乳杆菌ATCC 11741呈快速“S”型生长;产酸高峰期在2-7 h,14 h后趋于稳定,最终pH值稳定在4.3左右;该菌株在pH 4.0-11.0范围内生长良好,在pH 2.0的MRS培养基中培养4 h存活率为50.48%;在胆盐浓度为0.10%的MRS培养基中培养2 h之后的存活率可达到94.4400%;在NaCl浓度为6%的MRS培养基中仍能生长;温度对其生长影响显著,30-42℃促进生长,而20℃和50℃则抑制其生长;转速为120 r/min和180 r/min对其生长无显著影响;该菌株在5h时自聚集率为41.4%,疏水性(碳烃化合物黏着法)为44.5%。【结论】L.salivarius ATCC 11741是一株生长快,耐酸、耐碱、耐盐能力强,具有一定胆盐耐受性,温度适应性较广,具有较强黏附能力的乳酸菌。[Objective]Certain strains of Ligilactobacillus salivarius possess excellent probiotic properties and strong potential for application.This study characterized strain ATCC 11741 in terms of the growth characteristics,stress tolerance,and adhesion capacity,aiming to provide a theoretical basis for the utilization of L.salivarius.[Methods]L.salivarius ATCC 11741's accuracy was verified through morphological observations and 16S rRNA gene sequencing;its growth characteristics were investigated by tracking growth and acid production curves;additionally,a range of tolerance tests,including those with acid,alkali,bile salts,hyperosmolarity and temperature,were conducted to evaluate its tolerance;lastly,self-aggregation and hydrophobicity experiments were used to indirectly determine its adhesion.[Results]The growth curve of L.salivarius ATCC 11741 was formed like a“S”.The strain reached its highest acid production between 2–7 h,stabilizing at pH 4.3 after 14 h.After being cultured in the MRS medium at pH 2.0 for 4 h,the strain had a survival rate of 50.48%and grew well in the pH 4.0–11.0 range.Following 2 h of incubation in the MRS medium with 0.10%bile salt,the strain's survival rate was 94.4400%.In the MRS medium with 6%NaCl,the strain continued to proliferate.The growth of strain ATCC 11741 was significantly affected by temperature,being promoted at 30–42℃and inhibited at 20℃and 50℃.At the 5 h mark,the strain's hydrophobicity(measured by the hydrocarbon compound adhesion method)was 44.5%and its self-aggregation rate was 41.4%.[Conclusion]L.salivarius ATCC 11741 is a strain with fast growth,strong tolerance to acids,alkali,and salts,moderate tolerance to bile salt,a wide range of suitable temperatures,and strong adhesion capacity.

关 键 词：唾液宿主关联乳杆菌 生长特性 耐受效果 黏附能力 

分 类 号：TS201.3[轻工技术与工程—食品科学]