暹罗芽孢杆菌N-1对芒果果实采后贮藏品质和诱导抗病性的影响  

Effects of Bacillus siamensis N-1 on Storage Quality and Induced Disease Resistance of Postharvest Mango Fruits

作　　者：李佳怡 赵波 邵远志[3] 李雯 李蕊[2,3] 邹士成 LI Jiayi;ZHAO Bo;SHAO Yuanzhi;LI Wen;LI Rui;ZOU Shicheng(College of Food Science and Engineering,Hainan University,Haikou 570228,China;College of Tropical Agriculture and Forestry,Hainan University,Danzhou 571700,China;Sanya Nanfan Research Institute,Hainan University,Sanya 572025,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570228 [2]海南大学热带农林学院,海南儋州571700 [3]海南大学三亚南繁研究院,海南三亚572025

出　　处：《保鲜与加工》2024年第10期16-26,共11页Storage and Process

基　　金：海南省院士创新平台科研专项项目(YSPTZX202213);海南省院士团队创新中心建设项目(SQ2021YSPTJXRWS0074)。

摘　　要：为探究拮抗菌暹罗芽孢杆菌(Bacillus siamensis N-1)对芒果果实贮藏品质和抗病性的影响,以‘台农’芒果为试材,经过菌液浸泡处理后于25℃下贮藏,并对芒果果实采后贮藏品质、抗病相关酶活性及对应基因转录水平变化进行了分析。结果表明:与对照(蒸馏水处理)相比,B.siamensis N-1处理能够有效抑制‘台农’芒果病情指数的升高,减少丙二醛(MDA)积累,延缓果实硬度下降,保持较高的总黄酮含量,维持过氧化物酶(POD)、过氧化氢酶(CAT)、β-1,3-葡聚糖酶(GLU)、几丁质酶(CHT)、苯丙氨酸解氨酶(PAL)和查尓酮异构酶(CHI)活性。转录组数据分析表明共有9987个差异表达基因,主要富集在植物激素信号转导途径、代谢途径、光合作用途径、次生代谢物的生物合成途径以及光合作用-天线蛋白途径中。B.siamensis N-1显著诱导了PAL和WRKY转录因子的基因表达量的上调。综上,暹罗芽孢杆菌可有效维持芒果果实的贮藏品质,诱导果实抗病相关基因表达,抑制病害的发生。The present study aimed to investigate the effects of antagonistic bacterium Bacillus siamensis(B.siamensis N-1)on the storage quality and disease resistance of mango fruits.The‘Tainong’mango was used as the test material,and then stored at 25℃after the bacteria solution soaking treatment,and the changes of postharvest storage qualities,disease-resistance-related enzyme activities and gene transcription levels were analyzed.The results showed that compared with control group(distilled water treatment),the B.siamensis N-1 treatment could effectively inhibit the increase of disease index,reduce the accumulation of malondialdehyde(MDA),delay the decline of fruit firmness,maintain higher levels of total flavonoids contents,as well as the activities of peroxidase(POD),catalase(CAT),β-1,3-glucanase(GLU),chitinase(CHT),phenylalanine deaminase(PAL)and chalcone isomerase(CHI).Transcriptome data analysis showed that there were 9987 differentially expressed genes,which were mainly enriched in plant hormone signal transduction pathway,metabolic pathway,photosynthesis,secondary metabolite biosynthesis pathway and photosynthesis-antenna protein pathway.B.siamensis N-1 significantly induced the upregulation of PAL and WRKY transcription factor gene expressions.In conclusion,B.siamensis could effectively maintain the storage qualities of mango fruits,induce the expressions of disease-resistance related genes,and inhibit the occurrence of diseases.

关 键 词：芒果果实 拮抗微生物 暹罗芽孢杆菌N-1 贮藏品质 诱导抗病性 

分 类 号：S667.7[农业科学—果树学]