Efficient heterologous biosynthesis of verazine,a metabolic precursor of the anti-cancer drug cyclopamine,in Nicotiana benthamiana  

作　　者：Chengxi Kou Jingling Liu Xue Yin Di He Jia Liu Xin Hua Rui Ma Wei Sun Zheyong Xue Pengda Ma 

机构地区：[1]College of Life Sciences,Northwest A&F University,Yangling 712100,China [2]Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University),Ministry of Education,Harbin 150040,China [3]Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization,Northeast Forestry University,Harbin 150040,China [4]Jilin Provincial Key Laboratory of Agricultural Biotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China [5]Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Plant Communications》2024年第6期1-4,共4页植物通讯（英文）

基　　金：supported by the National Key Research and Development Program of China(2023YFA0915800);the Jilin Provincial Agricultural Science&Technology Innovation Project(CXGC202105GH).

摘　　要：Dear Editor,Among the large number of Veratrum steroid alkaloids(Zhang et al.,2020),cyclopamine has been shown to have various inhibitory mechanisms against cancer,including inhibition of the hedgehog signaling pathway,improvement of tumor tissue microenvironment,inhibition of cell respiration,promotion of cell apoptosis,and reversal of tumor drug resistance(Liu et al.,2023).The biosynthetic pathway of verazine,a possible metabolic precursor of cyclopamine,has been discovered in Veratrum californicum(Augustin et al.,2015).However,the content of Veratrum steroid alkaloids in plants is very low(Supplemental Figure 2),so reliance on direct extraction from plants for commercial production is not feasible.A more effective strategy is to synthesize rare plant secondary metabolites using synthetic biology technology(Liu et al.,2022).

关 键 词：CYCLOPAMINE CANCER 

分 类 号：Q94[生物学—植物学]