Functional mechanism study of the allelochemical myrigalone A identifies a group of ultrapotent inhibitors of ethylene biosynthesis in plants  

作　　者：George Heslop-Harrison Kazumi Nakabayashi Ana Espinosa-Ruiz Francesca Robertson Robert Baines Christopher R.L.Thompson Katrin Hermann David Alabadí Gerhard Leubner-Metzger Robin S.B.Williams 

机构地区：[1]Centre for Biomedical Sciences,Department of Biological Sciences,Royal Holloway University of London,Egham TW200EX,UK [2]Centre for Plant Molecular Sciences,Department of Biological Sciences,Royal Holloway University of London,Egham TW200EX,UK [3]Instituto de Biología Molecular y Celular de Plantas(CSIC-UPV),46022 Valencia,Spain [4]Centre for Life’s Origins and Evolution,Department of Genetics,Evolution and Environment,University College London,London,UK [5]Syngenta,Crop Protection AG,Stein,Switzerland

出　　处：《Plant Communications》2024年第6期246-262,共17页植物通讯（英文）

基　　金：supported by a PhD studentship funded by BBSRC DTP iCASE in collaboration with Syngenta Ltd.The CRISPR plasmids were kindly supplied by Dr.Yoichiro Kamimura,RIKEN Cell Signaling Dynamics Team,Center for Biosystems Dynamics Research,RIKEN(G90426).

摘　　要：Allelochemicals represent a class of natural products released by plants as root,leaf,and fruit exudates that interfere with the growth and survival of neighboring plants.Understanding how allelochemicals function to regulate plant responses may provide valuable new approaches to better control plant function.One such allelochemical,Myrigalone A(MyA)produced by Myrica gale,inhibits seed germination and seedling growth through an unknown mechanism.Here,we investigate MyA using the tractable modelDictyostelium discoideum and reveal that its activity depends on the conserved homolog of the plant ethylenesynthesis protein 1-aminocyclopropane-1-carboxylic acid oxidase(ACO).Furthermore,in silico modeling predicts the direct binding of MyA to ACO within the catalytic pocket.In D.discoideum,ablation of ACO mimics the MyA-dependent developmental delay,which is partially restored by exogenous ethylene,and MyA reduces ethylene production.In Arabidopsis thaliana,MyA treatment delays seed germination,and this effect is rescued by exogenous ethylene.It also mimics the effect of established ACO inhibitors on root and hypocotyl extension,blocks ethylenedependent root hair production,and reduces ethylene production.Finally,in silico binding analyses identify a rangeof highlypotentethylene inhibitorsthatblock ethylene-dependent responseand reduce ethyleneproduction in Arabidopsis.Thus,we demonstrate a molecular mechanism by which the allelochemical MyA reduces ethylene biosynthesis and identify a range of ultrapotent inhibitors of ethylene-regulated responses.

关 键 词：1-aminocyclopropane-1-carboxylic acid ACC ACC oxidase ACO ALLELOCHEMICALS ethylene synthesis inhibitors structure-activity relationship 

分 类 号：Q94[生物学—植物学]