Chlorinated Polyfluoroalkyl Ether Sulfonic Acids(Cl-PFESAs)Are Associated with Eye Diseases in Humans and Eye Toxicity in Zebrafish  

作　　者：Lu-Yin Wu Li-Xia Liang Yang Zhou Zeeshan Mohammed Zhengmin Min Qian Stephen Edward McMillin Maya Tabet Chu Chu Yuan-Yuan Fan Jia-Xin Zhou Jing-Wen Huang Wei-Hong Tan Guang-Hui Dong Li-Zi Lin 

机构地区：[1]Joint International Research Laboratory of Environment and Health,Ministry of Education,Guangdong Provincial Engineering Technology Research Center of Environmental Pollution and Health Risk Assessment,Department of Occupational and Environmental Health,School of Public Health,Sun Yat-sen University,Guangzhou 510080,China [2]State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment,South China Institute of Environmental Sciences,Ministry of Environmental Protection,Guangzhou 510655,China [3]Department of Developmental Biology&Genetics,School of Biological Sciences,Indian Institute of Science,Bangalore 400060,India [4]Department of Epidemiology and Biostatistics,College for Public Health&Social Justice,Saint Louis University,St.Louis,Missouri 63104,United States [5]School of Social Work,Saint Louis University,St.Louis,Missouri 63103,United States [6]College of Global Population Health,University of Health Sciences and Pharmacy in St.Louis,St.Louis,Missouri 63110,United States [7]Guangdong Cardiovascular Institute,Department of Reproductive Medicine,Department of Obstetrics and Gynecology,Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China [8]Department of Reproductive Medicine and Genetics Center,The People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China

出　　处：《Environment & Health》2024年第6期390-400,共11页环境与健康（英文）

基　　金：supported by the National Key Research and Development Program of China(2018YFC1004300,2018YFC1004301,and 2018YFE0106900);the National Natural Science Foundation of China(82173471,82003409,82103823,and 82073503);the Natural Science Foundation of Guangdong Province(2021A1515012212,2021A1515011754,2021B1515020015,2020A1515011131,2019A050510017,2018B05052007,and 2017A090905042);the Guangxi Key Research and Development Plan(GUIKEAB18050024).

摘　　要：Evidence from animal experiments has shown that chlorinated polyfluoroalkyl ether sulfonic acids(Cl-PFESAs)can induce vision dysfunction in zebrafish.However,environmental epidemiological evidence supporting this hypothesis remains limited.In our case−control study,samples collected from 270 individuals(135 controls and 135 cases)from the Isomers of C8 Health Project data were analyzed for Cl-PFESAs.We also repeated our analysis on zebrafish to support our findings in humans and to decipher the mechanism underlying Cl-PFESA eye toxicity.The serum levels of per-and polyfluoroalkyl substances(PFASs)and alternatives were significantly higher in the cases than in the controls.Higher serum Cl-PFESA levels were associated with greater odds of eye diseases,and the trend showed a statistically significant dose-dependent relationship.The Shapley additive explanations(SHAP)value indicated that 8:2 Cl-PFESA was the dominant eye disease risk factor among the 13 studied PFASs.In zebrafish experiments,Cl-PFESAs induced eye toxicity in adult zebrafish by oxidative damage and cell apoptosis.Compared to the control group,there was significantly reduced thicknesses of the inner plexiform layer(IPL),outer plexiform layer(OPL),and retinal tissue in the zebrafish exposed to Cl-PFESAs.Our study provides human clinical and animal experimental data,showing that exposure to PFASs increases the odds of the development of eye toxicity.

关 键 词：eye diseases chlorinated polyfluoroalkyl ether sulfonic acids(Cl-PFESAs) case−control study shapley additive explanations(SHAP) zebrafish experiment 

分 类 号：R77[医药卫生—眼科]