Chromatographic and mass spectroscopic guided discovery of Trichoderma peptaibiotics and their bioactivity  

作　　者：Adigo Setargie Chen Wang Liwen Zhang Yuquan Xu 

机构地区：[1]Biotechnology Research Institute,The Chinese Academy of Agricultural Sciences,12 Zhongguancun South Street,Beijing 100081,China [2]Institute of Biotechnology,Bahir Dar University,P.O.Box.79,Bahir Dar,Ethiopia

出　　处：《Engineering Microbiology》2024年第2期62-76,共15页工程微生物学（英文）

摘　　要：Peptaibiotics are linear or cyclic peptide antibiotics characterized by the non-proteinogenic amino acid,alpha-aminoisobutyric acid.They exhibit a wide range of bioactivity against various pathogens.This report presents a comprehensive review of analytical methods for Trichoderma cultivation,production,isolation,screening,pu-rification,and characterization of peptaibiotics,along with their bioactivity.Numerous techniques are currently available for each step,and we focus on describing the most commonly used and recently developed chromato-graphic and spectroscopic techniques.Investigating peptaibiotics requires efficient culture media,growth con-ditions,and isolation and purification techniques.The combination of chromatographic and spectroscopic tools offers a better opportunity for characterizing and identifying peptaibiotics.The evaluation of the chemical and biological properties of this compound has also been explored concerning its potential application in pharma-ceutical and other industries.This review aims to summarize available data on the techniques and tools used to screen and purify peptaibiotics from Trichoderma fungi and bioactivity against various pathogens.

关 键 词：CHROMATOGRAPHY peptaibiotics spectroscopy TRICHODERMA 

分 类 号：Q93[生物学—微生物学]